Characteristics of Acquired beta-lactamase Gene in Clinical Isolates of Multidrug-resistant Pseudomonas aeruginosa.
10.5145/KJCM.2008.11.2.98
- Author:
Sun Yang CHUNG
1
;
Ji Youn SUNG
;
Kye Chul KWON
;
Jong Woo PARK
;
Chi Seon KO
;
So Youn SHIN
;
Jeong Hoon SONG
;
Sun Hoe KOO
Author Information
1. Health Promotion Center, Samsung Medical Center, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Multidrug resistance;
Pseudomonas aeruginosa;
OXA type beta-lactamase;
Metallo-beta- lactamase (MBL)
- MeSH:
Anti-Bacterial Agents;
Bacterial Proteins;
beta-Lactamases;
Consensus;
Diffusion;
Drug Resistance, Multiple;
Epidemiologic Studies;
Humans;
Korea;
Mass Screening;
Oxytocin;
Polymerase Chain Reaction;
Prevalence;
Pseudomonas;
Pseudomonas aeruginosa;
Sequence Analysis, DNA
- From:Korean Journal of Clinical Microbiology
2008;11(2):98-106
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Recently, there have been reports of infections with multidrug-resistant Pseudomonas aeruginosa. To determine the mechanism of the resistance, we investigated the prevalence of Ambler class A and D beta-lactamases, their extended-spectrum derivatives, and class B and D carbapenemase in multidrug-resistant P. aeruginosa isolates. METHODS: During the period of March 2006 to May 2007, clinical isolates of multidrug-resistant P. aeruginosa were collected from patients in Chungnam National University Hospital, Daejeon, Korea. Inhibitor-potentiated disk diffusion tests were used for the screening of metallo-beta-lactamase (MBL) production. PCR and DNA sequencing were conducted for the detection of beta-lactamase genes. We also employed the enterobacterial repetitive intergenic consensus (ERIC)- PCR method for an epidemiologic study. RESULTS: A total of 37 consecutive, non-duplicate, multidrug-resistant P. aeruginosa were isolated. Twenty- nine of 37 isolates harbored blaOXA-10 (56.8%), blaOXA-2 (18.9%), and blaOXA-1 (5.4%). Only one isolate produced IMP-1, and it also harbored blaOXA-1. None harbored Ambler class A beta-lactamase or class D carbapenemase. The strains producing OXA type beta-lactamases showed a significantly higher resistance to aminoglycoside compared to non-producers. The ERIC-PCR pattern of the 19 OXA-10 producing strains indicated that the isolates were closely related in terms of clonality. CONCLUSION: OXA type beta-lactamases are the most prevalent among the acquired beta-lactamases produced by multidrug-resistant P. aeruginosa isolated at a university hospital in Chungcheong Province. Besides beta-lactam antibiotics, the strains harboring OXA type beta-lactamase showed a significantly higher resistance to aminoglycoside and qunolone.
