Study on the Anti-Inflammatory and Antifungal Effects of Taste-Masked Lithospermum Safflower Emulsion
10.14148/j.issn.1672-0482.2024.0812
- VernacularTitle:掩味型紫草红花乳膏剂抗炎抗真菌的作用研究
- Author:
Wenbo YUAN
1
;
Hongyao ZHONG
;
Xinyi CHENG
;
Kun WEI
;
Ying YAO
Author Information
1. 江南大学附属妇产医院,江苏 无锡 214002
- Keywords:
taste-masked Lithospermum Safflower emulsion;
antifungal;
anti-inflammatory;
Candida albicans
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2024;40(8):812-822
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the anti-inflammatory and antifungal action mechanism of taste-masked Lithospermum Saf-flower emulsion in vivo and in vitro.METHODS In vitro,the anti-inflammatory effect was detected by MTT assay,qPCR and ELISA.The anti-fungal effect of the product was investigated by broth dilution experiment,bactericidal kinetics,germ tube inhibition and XTT reduction test.In vivo,the effect was evaluated and the mechanism was investigated on the skin disease model of Candida al-bicans in mice.RESULTS Lithospermum in taste-masked Lithospermum Safflower emulsion had a significant inhibitory effect on the proliferation of RAW264.7 cells,and Safflower inhibited the production of IL-6 induced by LPS in a dose-dependent manner.Litho-spermum significantly inhibited the activity of Candida albicans,and its bactericidal mode is time-and concentration-dependent;Lithospermum significantly reduced the formation of Candida albicans germ tubes and destroyed its biofilm;Safflower had no direct kill-ing effect on Candida albicans,was not able to inhibit its biofilm formation,but significantly reduced the hyphal growth of Candida al-bicans and increased its ROS level.CONCLUSION The combination of Lithospermum and Safflower in the taste-masked Lithosper-mum Safflower emulsion can work synergistically to reduce inflammatory damage and treat Candida albicans infection of the skin.
