Serum Metabolomics Analysis of Baicalein for Prevention and Treatment of Radiation-Induced Intestinal Damage in Mice Based on GC-MS
10.14148/j.issn.1672-0482.2024.0702
- VernacularTitle:基于气质联用技术的黄芩素防治放射性肠损伤小鼠血清代谢组学分析
- Author:
Yejin ZHU
1
;
Xinping WANG
;
Qianyi WANG
;
Jun HE
;
Xingde ZHANG
;
Chao JIANG
Author Information
1. 南京中医药大学医学院,江苏 南京 210023
- Keywords:
radiation-induced intestinal injury;
baicalein;
GC-MS;
metabolomics;
metabolic network analysis
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2024;40(7):702-709
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the changes in serum metabolites of mice with radiation-induced intestinal injury under the intervention of baicalein and the changing characteristics of endogenous biological small molecules during the process of baicalein's participation in the prevention and treatment of radiation-induced intestinal injury through the metabolomics method based on GC-MS technology,in order to explore the potential regulatory mechanism of baicalein.METHODS A mouse radioactive intestinal injury model was established and randomly divided into normal control group,model group,low-dose baicalein group and high-dose baica-lein group.Baicalein was administered by gavage.Gas chromatography-mass spectrometry(GC-MS)technology was used to analyze the serum samples of mice in each group,and differential metabolites were screened through partial least squares-discriminant analysis(PLS-DA).Potential metabolic pathways were analyzed with MetaboAnalyst.RESULTS The pathological sections of mouse intesti-nal tissue showed that the high-dose and low-dose baicalein groups had a certain protective effect on radiation-induced intestinal dam-age.Metabolomic analysis showed that there were significant differences in the metabolic profiles of the blank control group,model group,low-dose and high-dose baicalein administration groups.After intragastric administration of baicalein,the endogenous metabo-lites in mice with radiation intestinal injury tended to normalize.The study screened out a total of 11 potential metabolic markers and 5 related metabolic pathways,among which pathways related to glucose metabolism,glutathione pathway,and ammonia metabolism were particularly significant.CONCLUSION Baicalein has a certain preventive and therapeutic effect on radiation-induced intestinal in-jury;baicalein participates in glucose metabolism and glutathione metabolism,and improving the endogenous substance disorder caused by radiation is its potential mechanism of action.
