Optimization of β-cyclodextrin Inclusion Process of Cinnamomi Ramulus Formula Granules Volatile Constituents Based on Standard Relation and Information Entropy Method
10.14148/j.issn.1672-0482.2024.0582
- VernacularTitle:基于基准关联度与信息熵权法优选桂枝配方颗粒中挥发性成分的β-环糊精包合工艺
- Author:
Yaxi CHEN
1
,
2
;
Zhuoyuan LI
;
Lin TAO
;
Nana CHEN
;
Yixin KE
;
Wen SHEN
;
Wei XIE
;
Wen ZHANG
;
Junsong LI
Author Information
1. 南京中医药大学药学院,江苏 南京 210023
2. 江苏省中药高效给药系统工程技术研究中心,江苏 南京 210023
- Keywords:
Cinnamomi Ramulus Formula Granules;
aromatic aqueous solution;
β-cyclodextrin;
information entropy method;
standard relation;
orthogonal design;
inclusion process
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2024;40(6):582-588
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To optimize β-cyclodextrin(β-CD)inclusion process of Cinnamomi Ramulus Formula Granules vola-tile constituents by orthogonal test,based on standard relation and information entropy method.METHODS On the basis of single factor experiments,the ratio of β-CD to aromatic aqueous solution,the inclusion temperature,and the inclusion time were selected as the investigating factors;the inclusion rate,drug loading,and standard relation of cinnamic aldehyde in inclusion complex were used as the evaluation index.The information entropy method was used to determine the weight coefficient of each index,then the comprehen-sive score was calculated,the inclusion process conditions were optimized by orthogonal experiment.The inclusion complex was charac-terized by thin layer chromatography,ultraviolet absorption spectroscopy,Fourier infrared spectroscopy,and X-ray diffraction.RE-SULTS The best inclusion process was that the ratio of β-CD to aromatic aqueous solution was 3∶100(g·mL-1),the inclusion temperature was 50℃,and the inclusion time was 1 h.The average inclusion rate of the obtained inclusion compound was 80.84%,the drug loading was 8.63%,and the standard relation was 0.91.The results of thin-layer chromatography,ultraviolet,infrared spec-troscopy and other characterization experiments showed that the volatile components in the aromatic aqueous solution successfully en-tered the β-CD cavity,and the inclusion complex was successfully prepared.CONCLUSION The optimum inclusion process is sta-ble and feasible,which can provide references for the preparation process of Cinnamomi Ramulus Formula Granules.
