UPLC-Q-TOF-MS Analysis on Chemical Constituents of Classical Prescription Xiehuang San Standard Decoction
10.14148/j.issn.1672-0482.2024.0569
- VernacularTitle:经典名方泻黄散标准煎液化学成分的 UPLC-Q-TOF-MS分析
- Author:
Wan XIAO
1
,
2
;
Siqi WANG
;
Jiazheng LI
;
Xuedan FU
;
Jianming JU
;
An KANG
;
Weifeng YAO
;
Hailang JIANG
Author Information
1. 南京中医药大学药学院,江苏 南京 210023
2. 江苏省中医药研究院中药制剂工程研究中心,江苏 南京 210028
- Keywords:
classical prescription;
Xiehuang San standard decoction;
UPLC-Q-TOF-MS;
chemical constituent
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2024;40(6):569-581
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To analyze the chemical constituents from classical prescription Xiehuang San(XHS)standard decoc-tion by UPLC-Q-TOF-MS technology,and classify the chemical composition and analyze the representative components.METHODS Acquity HSS T3 column(2.1 mm×100 mm,1.8 μm)was used as the chromatographic column,with 0.1%formic acid solution-0.1%formic acid acetonitrile as the mobile phase for gradient elution.The volume flow rate was 0.4 mL·min-1 and the column tem-perature was 40℃.Mass spectrometry data of XHS were collected in positive and negative ion modes.The chemical constituents from classical prescription XHS were analyzed and identified by Masslynx 4.1 software comparison with reference materials,mass spectrome-try data analysis and reference to relevant literature.RESULTS A total of 107 compounds were analyzed and identified from XHS,including 45 flavonoids,27 triterpenoids,11 monoterpenoids,10 phenylpropanoids,6 chromogenic ketones,5 alkaloids and 3 other other compounds.CONCLUSION The study provides an experimental basis for the further research on the substance basis and qual-ity control of XHS.
