Optimization of Composite Probiotics Fermentation Process for Notoginseng Radix et Rhizoma Residues by Box-Behnken Re-sponse Surface Method and Evaluation of Their in vitro Antioxidant Activities
10.14148/j.issn.1672-0482.2024.0555
- VernacularTitle:Box-Behnken响应面法优化复合益生菌发酵三七药渣工艺及体外抗氧化活性研究
- Author:
Yu WANG
1
;
Sheng GUO
;
Haifeng LIU
;
Dawei QIAN
;
Minggeng WANG
;
Lanping GUO
;
Jinao DUAN
Author Information
1. 南京中医药大学中药资源产业化与方剂创新药物国家地方联合工程研究中心/江苏省中药资源产业化过程协同创新中心/国家中医药管理局中药资源循环利用重点研究室,江苏 南京 210023
- Keywords:
Notoginseng Radix et Rhizoma residue;
probiotic;
analytical hierarchy process;
entropy weight method;
antioxidant ac-tivity in vitro
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2024;40(6):555-568
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE Notoginseng Radix et Rhizoma residues were used as raw material to compare the difference in the im-pact of different probiotics and composite probiotic on various indicator components through probiotic fermentation,aiming to explore the optimal fermentation process.METHODS The fermentation process was optimized using single factor and Box-Behnken response surface methodology,and the antioxidant capacity of the fermentation product was assessed through in vitro antioxidant experiments.RESULTS The results showed the optimum fermentation processes were 48 h of aerobic fermentation,36 h of anaerobic fermentation,ratio of 2∶3∶1 for Streptococcus thermophilus,Bifidobacterium bifidum and Lactobacillus acidophilus,solid-liquid ratio of 0.14 g·mL-1,inoculation quantity 5%,fermentation temperature 33℃.Under the optimal fermentation conditions,the content of neutral polysaccharide,acidic polysaccharide,and total flavonoid in Notoginseng Radix et Rhizoma residue increased by 105.64%,96.98%and 123.83%,respectively,which were high than those single-strain fermentation.The IC50 values of scavenging DPPH and ABTS free radicals in the fermentation products were 1.774 mg·mL-1 and 3.065 mg·mL-1,respectively,and the power of reducing Fe3+was 0.138 mmol FeSO4 g-1.The antioxidant capacity was significantly enhanced compared to the unfermented residues.CON-CLUSION The optimum fermentation process can significantly elevate the content of indicator components in Notoginseng Radix et Rhizoma residues and enhance its antioxidant capacity.Compared to single-strain fermentation,the content of various indicator compo-nents is significantly increased,showing no apparent antagonistic effect among the probiotics mentioned above.The study provides sci-entific evidence and data support for the resource utilization of Notoginseng Radix et Rhizoma residues.
