Study on the Anti-Fatigue and Intestinal Flora Improvement Effects of Fructan Products Isolated from Polygonatum Cyrtone-ma Hua
10.14148/j.issn.1672-0482.2024.0494
- VernacularTitle:多花黄精中果聚糖产物的抗疲劳与改善肠道菌作用研究
- Author:
Na WANG
1
;
Tao LIU
;
Chang MA
;
Xiaoxian YU
;
Jing JI
;
Lingchong WANG
;
Jianming CHENG
Author Information
1. 南京中医药大学药学院,江苏 南京 210023
- Keywords:
polysaccharide;
Polygonatum cyrtonema Hua;
anti-fatigue activity;
structural characterization;
intestinal flora
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2024;40(5):494-502
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the structural characteristics of purified polysaccharide isolated from Polygonatum cyrtonema Hua and explore its anti-fatigue and intestinal bacteria improvement effects,so as to provide theoretical basis for the development of products using this polysaccharide as functional ingredients.METHODS PMP pre-column derivatization-high performance liquid chromatography,trimethylsilyl ether derivatization-gas chromatography and other methods were used to chemically characterize the pu-rified polysaccharide;the mouse weight-loading swimming test and biochemical indicators were used to evaluate its anti-fatigue effect;16S rRNA intestinal flora sequencing was adopted to study the effect of the purified product in improving intestinal bacteria.RESULTS The obtained polysaccharide was a fructan product and contained small amounts of glucose(Glc),galactose(Gal),arabinose(Ara),mannose(Man),rhamnose(Rha)and galacturonic acid(GalA),which had a relative molecular mass in the range of 0.4-285 kDa,and the molecular weight of the main component was around 3 kDa.Compared with the model group,the high-dose polysaccha-ride group could significantly prolong the exhaustive swimming time of mice(P＜0.01),reduce the levels of BUN and UA in serum and the LA(P＜0.01)and MDA levels(P＜0.05)in muscle tissue,and enhance SOD(P＜0.01),GSH-Px(P＜0.001)and CAT(P＜0.05)levels in liver tissue.Compared with the blank control group,the low-dose polysaccharide group could increase the relative a-bundance of probiotics Eggerthelaceae(g_unclassified_f_Eggerthelaceae)and Weissella(g_Weissella)in the fecal microorganisms of mice(P＜0.05).CONCLUSION The chemical composition of purified polysaccharide isolated from Polygonatum cyrtonema Hua is determined,and it is proven that the polysaccharide has a good anti-fatigue effect and can promote the growth of probiotics in the intes-tine,which provides a basis for product development using this polysaccharide as a functional ingredient.
