Establishment and evaluation of a novel mouse model of orthotopic colon cancer in the mesenteric triangle of the cecum
10.3760/cma.j.issn.0253-3766.2015.06.004
- VernacularTitle:小鼠盲肠系膜三角原位种植结肠癌模型的构建与评价
- Author:
Xiangfeng HE
1
,
2
;
Wen SHI
;
Song WEN
;
Yongqiang SUN
;
Guojie LING
;
Kang SHEN
;
Chunlei PENG
;
Baoan CHEN
;
Jianhong WANG
Author Information
1. 210009 南京，东南大学医学院附属中大医院血液科
2. 226361 南通市肿瘤医院内科
- Keywords:
Colon neoplasms;
Neoplasms metastasis,liver;
Mouse model
- From:
Chinese Journal of Oncology
2015;(6):418-421
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of preparation of a mouse model of orthotopic colon cancer by injecting tumor cell suspension into mesenteric triangle of the cecum. Methods Twenty SPF 8?week old BALB/c mice ( male ∶ female=1 ∶ 1) were used in this study. The mouse caecum was exposed by laparostomy, and suspension of mouse colon adenocarcinoma CT26.WT cells was injected into the mesenteric triangle of cecum for preparation of a mouse model oforthotopic colon cancer. Results Mouse orthotopic colon cancer was developed by injection of tumor cell suspension into mesenteric triangle of the cecum showing a successful rate of 100%, without intestinal obstruction, and the liver, spleen, diaphragm and mesenteric lymph nodes metastasis rates were high in all the 20 experimental mice. Conclusions The establishment of mouse models of orthotopic colon cancer by injection of tumor cell suspension into the mesenteric triangle is a simple, rapid, and easy to master procedure, causing less damage to the colon wall, safe and with less trauma to the mice. This method may provide an ideal mouse model of orthotopic colon cancer for the study of pathogenesis as well as liver metastasis mechanisms of colon cancer.
