Study on the Multi-Component Skin Pharmacokinetics of Crossbow Microemulsion after Transdermal Administration Based on Microdialysis Combined with UPLC
10.14148/j.issn.1672-0482.2024.0167
- VernacularTitle:基于微透析结合UPLC的弩药微乳经皮给药后多成分皮肤药动学研究
- Author:
Huan XIE
1
;
Xuanfei SONG
;
Bin ZHOU
;
Yao LIU
;
Fangfang YANG
;
Yinglong CHEN
;
Yan ZHU
;
Yong-Ping ZHANG
Author Information
1. 贵州中医药大学药学院,贵州 贵阳 550025
- Keywords:
crossbow microemulsion;
microdialysis;
UPLC;
skin pharmacokinetics
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2024;40(2):167-173
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the pharmacokinetic characteristics of crossbow microemulsion in rabbit skin.METH-ODS The concentrations of chlorogenic acid,benzoyl new aconitine,periplocin and new aconitine were determined by UPLC.The effects of temperature,perfusion speed and mass concentration on the recovery rate of the probe were investigated by incremental and decrement methods,the changes of multi-component content in the dialysate at different time points after transdermal administration of crossbow microemulsion were determined by UPLC,and DAS 2.0 software was used to process the data.RESULTS The recovery rate of the probe increased with increasing temperature,and the higher the flow rate,the lower the recovery rate.The pharmacokinetic parameters of chlorogenic acid,benzoyl new aconitine,periplocin and new aconitine in microemulsion Cmax were(3.24±0.39)(1.04±0.29)(4.78±2.15)(2.86±0.55)μg·mL-1,respectively,and in AUC0-∞ were(52.42±30.84)(7.70±3.73)(42.23±13.93)(22.68±12.51)h·mg·mL-1,respectively.CONCLUSION A real-time in vivo microdialysis sampling technique was first used and a UPLC detection technique was established to study the skin pharmacokinetics of multi-components of crossbow microe-mulsion after transdermal administration,which provides a reference for the follow-up investigation and application of crossbow microe-mulsion.
