T umor a ngiogene sis promoted by fusion of glioma stem/proeg ni tor cells with bone marrow mesenchymals tem ec lls
10.3760/cma.j.issn.0253-3766.2015.05.004
- VernacularTitle:胶质瘤干祖细胞与骨髓间充质干细胞融合驱动肿瘤的血管形成
- Author:
Dongliang ZHAO
1
;
Xingliang DAI
;
Chao SUN
;
Jinsheng CHEN
;
Xiaoci RONG
;
Haiyang WANG
;
Qilong WANG
;
Qin RUI
;
Aidong WAGN
;
Zhongyong WANG
;
Jun DONG
;
Qing LAN
;
Qinag HUANG
Author Information
1. 215004,苏州大学附属第二医院神经外科
- Keywords:
Glioma;
Cell fusion;
Stem cell;
Mesenchymal stem cells;
Tumor
- From:
Chinese Journal of Oncology
2015;(5):336-341
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to clarify whether the fusion of bone marrow mesenchymal stem cells ( MSCs) with tumor cells can promote tumor angiogensis.Methods Human glioma stem/progenitor cells (GSPCs) (SU3 cells) were transfected with red fluorescent protein (RFP) gene.Bone marrow mesenchymal stem cells ( MSCs) were harvested from nude mice with whole-body green fluorescent protein (GFP) gene expression.Then the two kinds of cells were co-cultured in vitro.At the same time SU3-RFP was transplanted into the brain of GFP-expressing nude mice to establish xenograft tumors.The co-cultured cells, GFP/RFP double positive ( yellow ) cells and blood vessels obtained from the xenograft tumors were observed under fluorescent microscope and laser scanning confocal microscope.Results After five passages in vitro, MSCs maintained the proliferative activity and highly expressed CD105.CD105 was also expressed in the femurs of GFP-expressing nude mice, tumor cells, blood vessels of SU3 xenograft tumors, and clinical malignant gliomas.When MSCs were co -cultured with SU3-RFP, the ratio of yellow cells co-expressing RFP and GFP was significantly increased after extended time and continuous passages. According to the flow cytometry, yellow cells co-expressing RFP and GFP were 83.7%of the cultured cells. In tissue slices of the xenograft tumors, bundles of yellow vessel-like structure and cross-sectioned yellow vascular wall structures including vascular wall stroma cells were observed with RFP and GFP expression, and were identified as de novo formed vessels derived from fusion of MSCs with SU3-RFP cells.Conclu sion Cell fusion occurs between tumor cells and host MSCs and it promotes tumor angiogenesis.
