Research on Water Extraction Process of Flos Lonicerae-Fructus Forsythuae Based on Network Pharmacology and Design Space
10.14148/j.issn.1672-0482.2024.0047
- VernacularTitle:基于网络药理学结合设计空间的金银花-连翘药对水提取工艺研究
- Author:
Ting CUI
1
;
Meizhou LI
;
Lifan GAN
;
Jiaming LIN
;
Lijin LIANG
;
Xingpeng HUANG
;
Zhipeng ZHANG
Author Information
1. 广东一方制药有限公司/广东省中药配方颗粒企业重点实验室,广东 佛山 528244
- Keywords:
Flos Lonicerae-Fructus Forsythuae;
network pharmacology;
response surface method;
critical process parameters;
criti-cal quality attributes;
design space method
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2024;40(1):47-60
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To optimize the water extraction process of Flos Lonicerae-Fructus Forsythuae and determine the range of water extraction process parameters.METHODS The active components were screened by network pharmacology,and the indica-tor ingredients were determined in combination with the quality markers under the relevant terms of Chinese Pharmacopoeia 2020 edition and the literature.Take extraction yield and the extraction rate of the indicative component as the critical quality attributes of the water extraction process to screen critical process parameters.The mathematical model was established by Box-Behnken experimental design to investigate the interaction between CQAs and CPPs and build the design space of the water extraction process of Flos Lonicerae-Fruc-tus Forsythuae.RESULTS The extraction percentages of phenolic acids,forsythoside A and forsythin were screened as the index components;specifications of medicinal slices,extraction time and water addition were the key process parameters.Based on the estab-lishment and optimization of the design space,the optimum water extraction process was obtained as follows:the medicinal slice of Lian-Qiao was broken into 0.8-1.2 cm,adding 12 times the amount of water in the first and extract for 30-50 min,10 times the a-mount of water in the second and extract for 25-30 min.CONCLUSION The verification results show that the measured value ob-tained by using the design space method to optimize the water extraction process is close to the predicted value,indicating that the method is stable and reliable,which can provide ideas for its further process development and quality control for the couple medicines of Flos Lonicerae-Fructus Forsythuae.
