Effect of platelet derived growth factor-B and its receptor expression on the proliferation of renal cell carcinoma ACHN cells
10.3760/cma.j.issn.0253-3766.2015.03.003
- VernacularTitle:血小板衍生生长因子B及其受体的表达对肾癌ACHN细胞增殖的影响
- Author:
Wenling WANG
1
;
Zhenting ZHANG
;
Shuhua WANG
;
Jun DU
;
Xin YAO
Author Information
1. 300060,天津医科大学肿瘤医院泌尿生殖肿瘤科 国家肿瘤临床医学研究中心 天津市“肿瘤防治”重点实验室
- Keywords:
Subject words] Kidney neoplasms;
Cell line,ACHN;
Cell proliferation;
Platelet-derived growth factor-B;
Mouse
- From:
Chinese Journal of Oncology
2015;(3):170-174
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of platelet derived growth factor-B and its receptor expression on the proliferation of renal cell carcinoma ACHN cells in vitro and in vivo.Methods PDGF-B gene was transfected into human renal carcinoma cell line ACHN cells, and the proliferation capability of ACHN cells transfected with or without PDGF-B was assessed by MTT assay.The effect of PDGF-B on the expression of p-PDGFR-βin endothelial cells and vascular smooth muscle cells ( VSMC ) was detected by Western blot.ACHN cells transfected with PDGF-B were injected into mice ( untransfected ACHN as control) to induce tumor formation.Immunohistochemical staining was used to detect the expression of Ki-67 in tumor cells and the tumor volume was measured to compare the tumor growth in the two groups.Results The PDGF-B expression of ACHN cells in transfected group was significantly increased than that in the untransfected group.MTT assay showed that the proliferation capability of ACHN cells in the transfected and untransfected groups had no significant differences at different time points (P>0.05).The expression of p-PDGFR-βin VSMC was significantly increased when cultured with PDGF-B overexpression culture medium. The mean tumor size of the PDGF-B group and control group was (0.305 ±0.108) cm3 and (0.577 ± 0.218) cm3, respectively (P =0.007).Ki-67-positive tumor cells were (41.00 ±5.34)/HPF in the PDGF-B-transfected group and ( 5 5 .8 0 ±2 .9 5 )/HPF in the untransfected group ( P=0 .0 0 1 ) .Conclusion PDGF-B overexpression may up-regulate p-PDGFR-βexpression of VSMC in renal cell carcinoma, and inhibit the tumor cell proliferation and tumor growth through paracrine signaling.
