Association of polymorphism in the promoter region of PCA3 gene with risk of prosate cancer
10.3760/cma.j.issn.0253-3766.2015.02.006
- VernacularTitle:前列腺癌抗原3基因启动子短片段重复序列多态性与前列腺癌发生风险的关系
- Author:
Wu ZHOU
1
;
Zhihua TAO
;
Zhongyong WANG
;
Zhanguo CHEN
;
Mo SHEN
;
Qiyu XU
;
Haixiao XIE
;
Zhixian YU
;
Guorong CHEN
Author Information
1. 325005,温州医科大学附属第一医院医学检验中心
- Keywords:
Prostatic neoplasms;
Prostatic hyperplasia;
Prostate cancer gene antigen 3;
Short tandem repeat;
Polymorphism,single nucleotide;
Risk
- From:
Chinese Journal of Oncology
2015;(2):107-112
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the polymorphism in the promoter region of PCA3 gene and its relationship with risk of prostate cancer ( PCa) . Methods The promoter region of PCA3 gene of the DNA of peripheral blood mononuclear cells was detected by sequence analysis in the 186 PCa and 141 BPH patients and 135 healthy control individuals. If the samples were detected with polymorphism of insection/deletion, clone sequence analysis was used with pBS?T carrier to verify it. Results There were 5 polymorphisms. TAAA repeat times:4, 5, 6, 7, 8, and 8 genotypes (TAAA 4/5, TAAA 4/6, TAAA 5/5, TAAA 5/6, TAAA 5/7, TAAA 5/8, TAAA 6/6, and TAAA 6/7) were detected in the promoter region of PCA3 gene. The eight genotypes were divided into three groups: ≤10TAAA, 11TAAA, ≥12TAAA. Unconditional logistic regression analysis models were used to analyze the relationship between different genotypes and cancer risks adjusted by sex and age. The type 11TAAA and≥12TAAA was associated with higher relative risk for prostate cancer than the group ≤10TAAA [ OR=1. 74, 95%CI=1. 06?2. 87 ( for type 11TAAA);OR=5. 63, 95%CI=1. 85?17. 19 (for type≥12TAAA)]. In the 186 PCa patients, there was 62. 4% allele of PCA3 gene with AG/CA mutation found in the promoter 18?19 bp region of PCA3 gene and it had a close relation with the development of prostate cancer. Conclusions Short tandem repeats are found in the promoter region of the PCA3 gene in PCa patients, and the increase of TAAA repeat sequences highly enhance the relative risk of prostate cancer development. The occurrence of such STR might be related to the mutations in their upstream loci.
