Water Extract of Notopterygium Incisum Alleviates Neuropathic Pain by Regulating TRPV1
10.14148/j.issn.1672-0482.2021.0508
- VernacularTitle:羌活水提液通过TRPV1调节神经病理性疼痛镇痛机制研究
- Author:
Di-Jun WANG
1
;
Yong-Lan RUAN
;
Xiang JIN
;
Hai-Wang JI
;
Zong-Xiang TANG
;
Chang-Ming WANG
Author Information
1. 南京中医药大学医学院·整合医学院,江苏 南京 210023
- Keywords:
notopterygium incisum;
neuropathic pain;
DRG;
TRPV1
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2021;37(4):508-513
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the effect of water extract of notopterygium incisum (WN) on neuropathic pain and its molecular biological mechanism.METHODS Pain behavior test to detect the effects of WN on thermal hyperalgesia and me-chanical hyperalgesia in acute pain and chronic constriction injury(CCI)induced neuropathic pain model.Immunohistochemis-try and qPCR were used to detect the expression of TRPV1 .Calcium imaging was used to detect calcium influx of capsaicin (TRPV1 agonist) in CCI mice after oral administration of WN.RESULTS WN significantly reduced the thermal hyperalgesia and mechanical hyperalgesia in acute pain model and CCI model (P<0.001).The expression of TRPV1 in protein level and mRNA level in DRG neurons were significantly inhibited by administration of WN(P<0.05).The response of DRG neurons to capsaicin was significantly inhibited by the treatment of WN(P<0.001).CONCLUSION WN alleviated CCI-induced me-chanical allodynia and thermal hypersensitivity via modulating TRPV1 .Our results revealed a new molecular biological mecha-nism of analgesia effect to traditional Chinese medicine.
