Preliminary Study on Reversal of Sorafenib Resistance of Hepatocellular Carcinoma Cells by Huaier Granule
10.14148/ji.ssn.1672-0482.2020.0083
- VernacularTitle:槐耳颗粒逆转肝癌细胞对索拉非尼耐药的初步研究
- Author:
Zheng-Guang1 ZHANG
1
;
Bing1 LIU
;
Wei-Feng1 LIANG
;
Cun-Si2 SHEN
Author Information
1. 南京中医药大学医学院· 整合医学学院
- Keywords:
Huaier granule;
Sorafenib;
hepatocellular carcinoma;
drug resistance
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2020;36(1):83-87
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effect and related mechanism of Huaier granule on Sorafenib-resistant hepatocellular carcinoma cells BEL-7402/S. METHODS The proliferation-toxicity effect of Huaier granule on BEL-7402/S cells and the reversal multiple of drug resistance to Sorafenib were measured by CCK-8 assays. The effects of Huaier granule on the mRNA and protein levels of hypoxia inducible factor-lα(H7F-lα) and vascular endothelial growth factor (VEGF) in BEL-7402/S cells were detected by qPCR and Western blot, respectively. RESULTS Huaier granule inhibited the activities of BEL-7402/S cells, and partially reversed the resistance of BEL-7402/S cells to Sorafenib at the dose of no significant cytotoxicity, and the reversal multiple of drug resistance was 2.08;the results of qPCR showed that Huaier granule may down-regulate the mRNA level of HIF-lαin BEL-7402/S cells, but had no significant effect on the mRNA level of VEGF. The results of Western blot showed that Huaier granule may down-regulate the protein levels of HIF-lαand VEGF in BEL-7402/S cells. CONCLUSION Huaier granule may partially reverse the drug resistance of BEL-7402/S cells to Sorafenib, and its mechanism may be related to the decrease of the expression of H7F-lαand VEGF.
