Relationship between promoter methylation of Syk and Runx3 genes and postoperative recurrence and metastasis in gastric carcinoma
10.3760/cma.j.issn.0253-3766.2014.05.005
- VernacularTitle:抑癌基因脾酪氨酸激酶和人类Runt相关转录因子3基因启动子甲基化与胃癌术后复发转移的关系
- Author:
Huazhang HONG
1
;
Kai ZHOU
;
Ping FU
;
Qi HUANG
;
Jun WANG
;
Xihong YUAN
;
Jian LI
Author Information
1. 江西省人民医院干部外科
- Keywords:
Stomach neoplasms;
Syk kinase;
Runx3 protein;
DNA methylation;
Neoplasm recurrence;
Neoplasm metastasis;
Diagnosis;
Prognosis
- From:
Chinese Journal of Oncology
2014;(5):341-345
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between aberrant methylation of Syk and Runx3 genes and recurrence and metastasis after resection of gastric cancer . Methods Applying methylation-specific polymerase chain reaction technique , promoter methylation of Syk and Runx 3 genes in the tumor tissues and adjacent normal tissues of gastric cancer patients were detected to investigate the relationship between methylation status of the promoter region of Syk and Runx 3 genes and postoperative recurrence and metastasis . Results In the 70 cases of gastric cancer , the frequencies of promoter methylation of Syk and Runx3 genes were 45.7%(32/70) and 55.7% (39/70) in gastric cancer, and 0 (0/70) and 7.1%(5/70), respectively, in the adjacent normal tissues .The rates of promoter methylation of Syk and Runx3 genes in the gastric cancers were significantly higher than that in the adjacent normal tissues (P<0.001 for all).The promoter methylation of Syk and Runx3 genes was significantly correlated with the degree of tumor differentiation , depth of invasion , lymph node metastasis and pathological staging (P <0.05 for all).The frequency of postoperative recurrence and metastasis in 32 patients with Syk promoter methylation was 65.6% ( 21/32 ) and that in 38 cases with Syk promoter unmethylation was 18.4%(7/38), showing a significant difference between the two subgroups (χ2 =16.13, P<0.001).The rate of postoperative recurrence and metastasis in 39 patients with Runx3 promoter methylation was 61.5%(24/39) and that in 31 patients with Runx3 promoter unmethylation was 12.9% (4/31, P <0.001). Conclusions The methylation of Syk and Runx 3 promoters plays an important role in postoperative recurrence and metastasis of gastric cancer .Combined detection of promoter methylation of Syk and Runx 3 genes is helpful for early diagnosis and evaluation of prognosis of gastric cancer .
