Investigation on the Anti-Metastatic Effect of Alisol B Isolated from Alismaorientale on 4T1 Breast Cancer Cells in Vitro
10.14148/j.issn.1672-0482.2018.0178
- VernacularTitle:泽泻醇B抗4T1乳腺癌细胞侵袭转移的体外研究
- Author:
Ai-Feng ZHANG
1
;
Yu-Qing SHENG
;
Ming-Chang ZOU
Author Information
1. 镇江市第四人民医院药剂科
- Keywords:
Alisol B;
breast cancer;
Alismaorientale;
metastasis;
matrix metalloproteinases
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2018;34(2):178-180
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the anti-metastatic effect of Alisol B on 4T1 breast cancer cells and explore its possi-ble mechanisms.METHODS 4T1 breast cancer cells were treated with Alisol B for 24 h.Cell viability was evaluated by MTT assay.Migration ability was evaluated by wound healing assay.Invasion ability was investigated by transwell invasion assay. The expressions of matrix metalloproteinases(MMP-2 and MMP-9)were detected by Western blot analysis.RESULTS Ali-sol B showed inhibitory effect on cell growth in 4T1 cells.Wound healing assay demonstrated that Alisol B inhibited the migra-tion of 4T1 cells after the treatment for 24 h.The migration rate was(52.66±8.48)%(P<0.01).Transwell invasion assay proved that Alisol B effectively suppressed the invasion of 4T1 cells.The invasion rate was(47.06 ± 9.32)%(P<0.01). Western blot results demonstrated that the expression of MMP-2 and MMP-9 were significantly down-regulated with the treat-ment of Alisol B.CONCLUSION Alisol B can suppress the metastasis of 4T1 breast cancer cells in vitro,which could be through down-regulation the expression of matrix metalloproteinases.
