The Anti-Proliferative Activity and Mechanism Exploration of Xiao'ai Jiedu Decoction on Breast Cancer Cell Lines MDA-MB-2 3 1 Through PI3K/AKT Pathway
10.14148/j.issn.1672-0482.2018.0173
- VernacularTitle:消癌解毒方通过调控PI3K/AKT通路抑制乳腺癌细胞株MDA-MB-231增殖的作用与机制探讨
- Author:
Hao SUN
1
;
Wei-Xin SHENG
;
Chang-Liang XU
;
Dong-Dong SUN
;
Jia-Ni TAN
;
Hui-Qin XU
;
Hai-Bo CHENG
Author Information
1. 南京中医药大学药学院
- Keywords:
Xiao'ai Jiedu decoction;
breast cancer cells;
cell proliferation;
PI3K/AKT pathway
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2018;34(2):173-177
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the anti-proliferative activity and mechanism of Xiao'ai Jiedu decoction against breast cancer cell lines MDA-MB-231.METHODS The cell morphology was observed by microscope.MTT method was used to in-vestigate the inhibitory rate of Xiao'ai Jiedu decoction against breast cancer cell lines MDA-MB-231.Flow cytometry analyzed the cell cycle distribution.Western blot detected the expression of Cyclin B1 and CDK1 and the expression of upstream signa-ling molecules P53,P21 in PI3K/AKT signaling pathway.RESULTS Compared with the control group,MDA-MB-231 cells of Xiao'ai Jiedu decoction group showed number-decreased,loosely arranged,morphology-smaller and rounder.Xiao'ai Jiedu decoction inhibited the proliferation of MDA-MB-231 cells(P<0.05,P<0.01).Xiao'ai Jiedu decoction down-regulated the ex-pression of Cyclin B1 and CDK1,inhibited the PI3K/AKT signaling pathway,and up-regulated the expression of P53 and P21 (P<0.05,P<0.01).CONCLUSION Xiao'ai Jiedu decoction can inhibit the proliferation of MDA-MB-231 breast cancer cells,perhaps related to inhibiting the PI3K/AKT signaling pathway.
