Protective Effect of Bushen Yiqi Huoxue Fang on Retinal Oxidative Damage in Model Mice of Dry Age-Related Macular Degenera-tion
10.14148/j.issn.1672-0482.2018.0162
- VernacularTitle:补肾益气活血方对干性年龄相关性黄斑变性模型小鼠视网膜氧化损伤的保护作用
- Author:
Jing XIE
1
;
Min LI
;
Xin-Rong XU
;
Li HANG
Author Information
1. 南京中医药大学附属医院
- Keywords:
age-related macular degeneration;
model mice;
Bushen Yiqi Huoxue Fang;
oxidative damage;
Nrf2 pathway
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2018;34(2):162-167
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE The study was aimed at evaluating protective effect of Bushen Yiqi Huoxue Fang on retinal oxida-tive damage in model mice of dry age-related macular degeneration(AMD)and to elucidate the underlying mechanism.METH-ODS 60 six-month-old C57BL/6 female mice,10 were fed normal diet as aging control,50 were fed a high-fat diet for 6 months followed by HQ(0.8%)in the drinking water for 3 months to established dry AMD model mice.Model mice were di-vided randomly into vehical control,positive drug(lutein)and 3 treatment groups(dosage:low,middle,high).Drugs were giv-en once-daily through gastrogavage for 3 months.At the end of the experimental period,the mice were killed and the eyes im-mediately removed.Transmission electron microscopy were used to evaluate sub-RPE deposit formation and Bruch membrane (BrM)thickness;The serum were separated from the blood sampling of mice eye vein,mice retina were detached and homog-enated,the serum and retina SOD,GSH-PX,CAT enzyme activity and ROS,MDA content were detected;The mRNA and protein expression of Nrf2,HO-1,NQO-1,GCL were detected by qPCR and Western blot,respectively.RESULTS Com-pared with aging control mice,the sub-RPE deposits was significantly increased,and the Bruch membrane was significantly thickened in vehical control mice;Bushen Yiqi Huoxue Fang can significantly inhibit sub-RPE deposit formation and reduce thickening of BrM in model mice compared to the vehical control mice,the sub-RPE deposits was decreased,BrM thickness was inhibited in treatment mice.Bushen Yiqi Huoxue Fang could increase SOD,GSH-PX and CAT enzyme activity,reduce ROS,MDA content of retina and serum in mice.mRNA and protein expressions of Nrf2,HO-1,NQO-1,GCL of HO-1, NQO-1 were up-regulated in mice treated with Bushen Yiqi Huoxue Fang(middle,high dosage).CONCLUSION Bushen Yiqi Huoxue Fang could protect the retina against oxidative damage in model mice of dry AMD,the underlying mechanism may be to increase SOD,GSH-PX and CAT enzyme activity and up-regulate expressions of Nrf2,HO-1,NQO-1,GCL through activa-tion of Nrf2 pathway,which may strengthen the clearance of reactive oxygen species in model mice.
