Effects of Gubi Recipe on the PI3K/AKt Pathway and Synovial Tissue of Knee Joint in the Rats Model of Knee Osteoarthritis
10.14148/j.issn.1672-0482.2018.0157
- VernacularTitle:骨痹方对大鼠膝骨关节炎PI3K/AKt信号通路及组织形态学的影响
- Author:
Xiao-Jin HE
1
;
Lei WANG
;
Rui-Rui WANG
;
Yi-Jiang QIU
Author Information
1. 南京中医药大学附属医院
- Keywords:
KOA;
Gubi recipe;
cartilage cells apoptosis;
phosphoinositide 3 kinase/protein kinase B
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2018;34(2):157-161
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the effects of Gubi recipe on the PI3K/AKt pathway and synovial tissue of nnee joint in the rats model of knee osteoarthritis.METHODS SD rats(n=40)were duplicated by Hulth's technique and randomly divided into the model group,the Glucosamine Sulfate capsules group,the low dose group of Gubi recipe,the dose group of Gubi reci-pe and the high dose group of Gubi recipe.Also,the rats in the sham group(n=8)were exposed to the cruciate ligament after the operation,and the normal group(n=8)did not need any disposal.All rats were given the solution by filling the stomach for 8 weeks.8 weeks later,the the synovial membrane was taken from each rat for observing;Western blot technic of articular cartilage PI3K/AKt pathway AKt activation extent and detect the expression of Bcl-2 and Bax.RESULTS Gubi recipe can im-prove the knee synovial membrane injury score of rats,effectively reduce the inflammatory reaction in knee synovial mem-brane.The expression of AKt and downstream target protein Bcl-2 was lower than that of normal group,the expression level of AKt in the high dose group of Gubi recipe was much higher than that in the normal group(P<0.01),the expression level of AKt in the middle dose group was close to that in the normal group;compared with the model group,the expression of AKt and the downstream target protein Bcl-2 in the high dose group were significantly higher(P<0.01),and the expression of Bax was decreased.CONCLUSION Gubi recipe can effectively reduce the chondrocyte apoptosis of osteoarthritis of the knee le-sions in the process,to some extent improve the pathology of knee osteoarthritis,the mechanism may be related to the inter-vention of PI3K/AKt pathway of knee osteoarthritis cartilage tissue of rat.
