Clonality analysis of intraductal proliferative lesions in the mammary gland

VernacularTitle:乳腺导管内增生性病变的克隆性分析
Author: Hua LI ¹ ; Qi CHEN ; Rong ZHU ; Dong-Hua GU ; Hong-Guang ZHU
Author Information

1. 复旦大学上海医学院
Keywords: Intraductal proliferative lesions; Clonality; X-inactivation HUMARA assay
From: Chinese Journal of Oncology 2008;30(1):55-58
CountryChina
Language:Chinese
Abstract: Objective To detect the clonal origin of intraductal proliferative lesions in the mammary gland. Methods Lesional and normal breast gland cells were microdissected from paraffin-embedded tissues using a laser capture microdissection (LCM) system. The genomic DNA was extracted. After digestion by restriction enzyme Hpa II, human androgen receptor (HUMARA) exonl was amplified by a fluorescent nested-PCR procedure and the PCR products were separated by a DNA sequencer and the fluorescent intensity of the two HUMARA alleles was analyzed. Results DNA from 88 of 101 (87.1%) patients could be amplified at the HUMARA locus and 68 of them (77.3%) were heterozygous and informative. 9/12 usual ductal hyperplasia (UDH) and 5/18 ductal intraepithelial neoplasia (DIN) 1A showed a polyclonal inactivation. 3/12 UDH, 13/18 DINIA, 28/28 DIN1B, 10/10 carcinoma in situ were monoclonal origin. Conclusion DIN 1A, 1B and carcinoma in situ are of monoclonal origin and real tumors.