The study on DNA methylation of p53-bax mitochondrial apoptosis pathway in cholangiocarcinoma
10.3321/j.issn:0253-3766.2008.01.013
- VernacularTitle:胆管癌p53-bax线粒体凋亡通路的甲基化研究
- Author:
Xiao-Fang LIU
1
;
Yong-Liang DUAN
;
Fan-Min KONG
;
Zheng XU
;
Xian-Ting ZHOU
;
Cui-Sheng ZHANG
;
Shao-Jun LI
Author Information
1. 青岛大学医学院附属烟台毓璜顶医院
- Keywords:
Cholangiocarcinoma;
Methylation specific PCR;
p53-bax mitochondrial apoptosis pathway
- From:
Chinese Journal of Oncology
2008;30(1):51-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical significance of gene methylation of p53-bax mitochondrial apoptosis pathway in the carcinogenesis of cholangiocarcinoma. Methods Promoter hypermethylation of DAPK, P14ARF and ASC genes were detected by methylation-specific PCR. Exon 5-8 of p53 gene were examined by automatic sequencing. Results It was found that 66.7% of 36 cholangiocarcinoma patients had methylation of at least one tumor suppressor gene. The rate of tumor suppressor gene methylation in these cholangiocarcinomas was 25.0% in P14FRF, 30.6% in DAPK and 36.1% in TMS1/ASC. The methylation rate of tumor suppressor gene in tissues adjacent to the cancer tissue was 13.9% including 5.6% in DAPK and 8.3% in TMS1/ASC. p53 gene mutation was detected in 22 of 36 patients(61.1%). Fourteen patients (38.9%)was found to have p53 gene mutation associated with the methylation of tumor suppressor gene. The rate of p53 gene mutation and methylation of tumor suppressor gene were statistically and significantly correlated with the features of tumor biology including differentiation and invasion (P< 0.05). Conclusion DNA methylation of p53-bax mitchondrial apoptosis pathway may be a frequent molecular event in the carcinogenesis of cholangiocarcinoma. Although the methylation rate of ASC, DAPK genes is relatively low, it may be helpful for early diagnosis, p53 gene mutation associated with the methylation of tumor suppressor genes may be correlated with tumor malignant biologic features.
