Expression and clinical significance of ezrin mRNA in human primary sporadic colorectal carcinoma
10.3321/j.issn:0253-3766.2008.01.009
- VernacularTitle:散发性结直肠癌组织中ezrin mRNA的表达及临床意义
- Author:
Ge YAN
1
;
Xiao-Yan ZHOU
;
Ye-Jun QIN
;
Xiu-Ying XIAO
;
Gui-Hong ZHANG
;
Xiang DU
Author Information
1. 上海交通大学医学院附属第一人民医院
- Keywords:
Colorectal neoplasms;
Ezrin mRNA;
Ezrin;
Immunohistochemistry;
Realtime RT-PCR
- From:
Chinese Journal of Oncology
2008;30(1):34-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression pattern of membrane-cytoskeleton linker ezrin mRNA in human sporadic colorectal carcinoma (SCRC), and to evaluate the correlation of ezrin mRNA expression with carcinogenesis, progression and metastasis of SCRC. Methods TaqMan real-time reverse transcription polymerase chain reaction (real-time RT-PCR) technique and immunohistochemical (IHC). EnVision staining were used to detect the expression of ezrin mRNA and ezrin protein in 132 SCRC specimens and 43 adjacent normal eolorectal mucosa specimens, including 74 cases with metastasis and 58 without. The correlation between the expression of ezrin mRNA and the process of carcinogenesis and metastasis in SCRC was analyzed, and the correlation between the location of ezrin expression and the clinicopathological parameters was also analyzed. Results The expression of ezrin mRNA and ezrin protein expression rate were significantly higher in SCRC than that in adjacent normal eolorectal mucosa (P< 0.05) ; which was also significantly higher in the metastatic group than that in the non-metastatic group (2.3580±0.5584 vs. 2.1129±0.6625, P=0.037;86.5% vs. 70.7%, P=0.026). The rate of ezrin protein expression on the membrane was 31.1% in the metastatic group versus 6.9% in the non-metastatic group (P<0.001), but ezrin mRNA expression was only slightly higher in the metastatic group (2.2671± 0.5990) than that in the non-metastatic group (2. 2448±0. 6241). No significant difference was found in cytoplasmic ezrin expression rate between the metastatic group (55.4%) and non-metastatic group (63.8% ). Ezrin mRNA expression was significantly higher in the group at age of < 50 years (2.5165±0.5755 ) than that at age of ≥50 years (2.1762±0.6094, P=0.016). The rate of ezrin protein expression on the membrane in the right colon was significantly higher than that in the left colon and rectum ( P=0.0003 ), but rate of cytoplasmic ezrin protein expression in the left colon was higher than that in the right colon and rectum(P=0.004). No correlation was found between the expression level of ezrin mRNA and the age, sex, tumor size, location, differentiation and depth of invasion. Conclusion The expressions of ezrin mRNA and ezrin protein were closely correlated with the process of carcinogenesis, progression and metastasis in SCRC, and the ezrin protein expression is consistent with expression trend of ezrin mRNA. The switching of ezrin localization from the cytoplasm to the membrane may play an important role in metastasis. The expression of ezrin mRNA is correlated with patients'age; membrane, and cytoplasmic ezrin expression is related to the tumor location. Ezrin may potentially become an important parameter in determining biological behavior of SCRC.
