Expression of PAK4 and its significance in gastric cancer
10.3321/j.issn:0253-3766.2008.01.008
- VernacularTitle:p21活化激酶4在胃癌中的表达及意义
- Author:
Ze LI
1
;
Hui-Mian XU
;
Feng LI
Author Information
1. 吉林省肿瘤医院
- Keywords:
Stomach neoplasms;
PAK4
- From:
Chinese Journal of Oncology
2008;30(1):30-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of p21-activated kinase4 (PAK4) in human gastric cancer, and to evaluate the correlation of PAK4 protein level with clinical pathologic parameters. Methods Immunohistochemical method was employed for detecting the expression of PAK4 in tissue samples obtained from 95 gastric cancer patients who underwent surgical treatment. Western blot was also used to measure the expression of PAK4 in gastric cancer tissues and adjacent non-cancerous gastric mucosa in 40 patients and also in 7 human gastric cancer cell lines. Results Immunohistochemical staining results showed that positive PAK4 expression in the gastric cancer tissues was 67.4%, and 96.9% of those (62/64) was located in the cytoplasm, while only 3.1% (2/64) was on the cell membrane. Expression of PAK4 was revealed to be significantly and positively correlated with both TNM stage (X2=10. 426, P<0.01) and lymph node metastasis (X2= 4.912, P<0.05 ). Western blot showed that PAK4 expression of gastric cancer tissues was significantly higher than that of adjacent non-cancerous gastric mucosa (t=-5. 978, P<0.01). Expression of PAK4 could also be detected in 7 human gastric cancer cell lines. Compared with GES-1, expression of PAK4 is higher in MKN45, AGS, N87 and BGC823, but it was lower in MGC803, MKN1 and SGC7901. Conclusion The overexpression of p21-activated kinase4 (PAK4) may be correlated with the carcinogenesis and prognosis of gastric cancer.
