Effects of Ca2+-binding protein S100A6 on Wnt/β-catenin signaling pathway
10.3321/j.issn:0253-3766.2008.01.004
- VernacularTitle:钙结合蛋白S100A6对Wnt/β-catenin信号途径的影响
- Author:
Tian-Xia LAI
1
;
Jing-Kun MIAO
;
Huan-Ling HE
;
Guo-Wei ZUO
;
Xing-Xing LI
;
Yan WANG
;
Sheng WANG
;
Tong-Chuan HE
;
Lan ZHOU
Author Information
1. 第三军医大学附属西南医院
- Keywords:
S100A6;
Wnt/β-catenin signaling pathway;
Protein-protein interaction
- From:
Chinese Journal of Oncology
2008;30(1):12-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the effects of S100A6 on Wnt/β-catenin signaling pathway and its molecular mechanism. Methods The expression of GST-hS10OA6 was induced with IPTG in Escherichia coli BL21, and the fusion protein was purified with glutathione-sepharose 4B beads. β-catenin level of human colon cancer cell line MG63 and human osteosarcoma cell line HCTl16 cells infected with AdS10OA6 was measured by Western blot. Luciferase activity assay was applied to analyze the effect of S100A6 on the β-catenin/TCF4 activity. The interactions between S100A6 and β-catenin/GSK-3β/Dvl/Axin were detected by GST-pulldown/Western blot. Results The β-catenin level in AdS100A6-infected MG63 and HCT116 cells was significantly increased in comparison with that in the AdGFP control group (P<0.01). The luciferase activity in human embryonic renal cell line 293 cells transfected with pTOP-Luc and followed by GST-hS100A6 treatment was increased by 20. 2-fold in comparison with that in the GST control group (P<0.01). The interaction between GST-hS100A6 and Axin was not found. Conclusion S100A6 up- regulates the Wnt/β-catenin signaling pathway, and this may be attributed to the interaction between S100A6 and β-catenin/GSK-3β/Dvl.
