Determination of Lithospermoside and Griffonilide in Rat Plasma by UPLC-MS/MS
10.14148/j.issn.1672-0482.2018.0091
- VernacularTitle:UPLC-MS/MS法测定大鼠血浆中紫草氰苷、格列风内酯浓度
- Author:
Hui-Sen FAN
1
;
Qiu-Ying YAN
;
Hai-Bing ZHANG
;
Hai-Bo CHENG
;
Wei-Xing SHEN
;
Zhen-Zhong WANG
;
Dong-Dong SUN
Author Information
1. 南京中医药大学转化医学研究中心
- Keywords:
Semiaquilegiae radix;
lithospermoside;
griffonilide;
UPLC-MS/MS;
pharmacokinetics
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2018;34(1):91-95
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish an UPLC-MS/MS method for the determination of lithospermoside and griffonilide in rat plasma and study its pharmacokinetic characteristics.METHODS The separation was performed on Aglient C18 column (100 mm × 2.1 mm,1.8 μm) with acetonitrile (A) and 0.1％ aqueous formic acid (B) as the gradient elution mobile phase (0 ～1 min,5％～20％B;1～2min,20％～60％B;2～6min,60％～70％B;6～6.Smin,70％～5％B;6.5～7.5min,5％B),the flow rate was 0.3 mL/min,the column temperature was set at 35 ℃ and the injection volume was 3 μL.UPLC-MS/MS was used to detected the concentration of lithospermoside and griffonilide in rat plasma after oral administration of Semiaquilegiae Radix.The pharmacokinetics parameters of lithospermoside and griffonilide were calculated by DAS 2.0 software.RESULTS Good linearity was found in lithospermoside and griffonilide within linear range from 0.701 9 ～ 140.4 ng/mL and 1.120 2 ～ 240.4 ng/mL,respectively.The RSD of accuracy and intra-/inter-day precision were less than 15％.The recovery of the method was ranged from 87.22 ％ to 107.75 ％.Lithospermoside and griffonilide were absorbed rapidly,the highest plasma concentration reached at 0.5 h,and the drug removed from body very fast and eliminated completely in 24 h.CONCLUSION This method is accuracy and stability for the study on pharmacokinetics of lithospermoside and griffonilide in rat plasma.
