Gancao Xiexin Decoction Ameliorates TNBS/Ethanol-Induced Ulcerative Colitis in SD Rats Through Regulation of the IL-6/STAT3 Signaling Pathway
10.14148/j.issn.1672-0482.2017.0627
- VernacularTitle:基于IL-6/STAT3信号通路研究甘草泻心汤治疗溃疡性结肠炎的作用机制
- Author:
Hao CHEN
1
;
Su XU
;
Shuai YAN
;
Bing-Xue CHEN
;
Bo ZHANG
;
Qin LU
;
Hui SUN
;
Xing-Xin ZHOU
;
Li ZENG
Author Information
1. 东南大学附属中大医院
- Keywords:
ulcerative colitis;
Gancao Xiexin decoction;
interleukin-6;
signal transducer and activator of transcription 3
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2017;33(6):627-632
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To observe the curative effect of Gancao Xiexin Decoction(GCXXT)on ulcerative colitis(UC) model rats and explore the regulation mechanism of related molecules in the pathways of IL-6/STAT3.METHODS 60 SD rats were randomly divided into 6 groups:control,TNBS/ethanol,mesalazine and GCXXT(2.93,5.86,11.72 g/kg)groups. Blood serum was extracted to detect the content of ALT,AST,BUN and Cr.Colon tissues were observed by optical micro-scope.The expressions of protein of IL-6,STAT3 and phosphate STAT3 were evaluated by immunohistochemistry.The ex-pressions of mRNA of IL-6 and STAT3 were analyzed by qPCR.RESULTS Compared with the blank group,AST in serum of model group increased obviously(P<0.01).However,ALT,BUN and Cr displayed no change(P>0.05).Pathological scores of colon tissues of model group increased significantly(P<0.01).The expressions of protein and mRNA of IL-6 and STAT3 in colon tissues increased in model group(P<0.01).Furthermore,the activation of STAT3 increased(P<0.01). Compared with the model group,AST in serum of rats of three groups treated with GCXXT decreased(P<0.01).Pathologi-cal scores of colon tissues of mesalazine group and medium dose GCXXT group decreased significantly(P<0.01).After the treatment of mesalazine or GCXXT,the expression of protein of IL-6,STAT3 and phosphate STAT3 reduced(P<0.01). The expression of mRNA of IL-6 and STAT3 decreased(P<0.01).CONCLUSION GCXXT can alleviate the pathological changes of colon,and medium dose of it can lead to satisfactory results.The mechanism may be related to its abilities to de-crease expression of IL-6 in colonic tissue,inhibit IL-6/STAT3 signaling pathway and reduce the expression and activation of STAT3.These results showed that GCXXT is effective and safe in ameliorating ulcerative colitis.
