Sulfotanshinone ⅡA Sodium Ameliorates Lipopolysaccharide-Peritoneal Dialysis Solution-Elicited Human Peritoneal Mesothelial Cells Injury
10.14148/j.issn.1672-0482.2017.0603
- VernacularTitle:丹参酮ⅡA磺酸钠注射液改善脂多糖-高糖腹膜透析液诱导的人腹膜间皮细胞损伤的实验研究
- Author:
Yao ZHOU
1
;
Kun GAO
;
Ping XIA
;
Wei LI
;
Zhan-Wei ZHOU
;
Min-Jie WANG
;
Wei SUN
;
Li-Jun WANG
;
Wei-Ming HE
Author Information
1. 南京中医药大学第一临床医学院
- Keywords:
peritoneal dialysis;
human peritoneummesothelial cells;
primary cell cultured;
sulfotanshinone ⅡA sodium in-jection;
peritoneal fibrosis
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2017;33(6):603-607
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the effect and mechanism of sulfotanshinone ⅡA sodium(STS)in human peritoneal mesothelial cells(HPMCs)injury and peritoneal fibrosis caused by lipopolysaccharide-peritoneal dialysis solution(LPS-PDS). METHODS HPMCs was primary cultured from patients undergoing peritoneal surgery.The cells were incubated with LPS-PDS to mimic the peritoneal dialysis conditions in vitro.After treatment of cells with STS,cellular viability was tested and mRNA were collected and subjected to RT-PCR to evaluated the level of TGF-β1,TIMP-1 and MMP-9.RESULTS Incubation HPMCs with LPS-PDS elicited cell injury.STS attenuated LPS-PDS-induced cell injury by improvement of cellular viability. Furthermore,STS inhibited HPMCs fibrosis as evidenced by suppression of TGF-β1 and TIMP1 induced by LPS-PDS and in-creasing MMP-9 mRNA level.CONCLUSION STS protects HPMCs against LPS-PDS-induced cell injury.Moreover,STS re-tards HPMCs fibrosis by decreasing TGF-β1 and TIMP1 mRNA level and increasing MMP-9 mRNA.
