Effect of Bushen Zhuyun Formula on Pituitary GnRH Receptor and Signal Transduction System in LPD Rats
10.14148/j.issn.1672-0482.2017.0554
- VernacularTitle:补肾助孕方对LPD模型大鼠垂体GnRH受体信号转导系统的影响
- Author:
Bo-Ru ZHOU
1
;
Hui-Fang ZHOU
;
Bei LIU
;
Hong-Kun ZHU
;
Jia-Xuan DAI
;
Jian-Ya XU
;
Tong XIE
Author Information
1. 南京中医药大学第一临床医学院
- Keywords:
Bushen Zhuyun formula;
luteal phase defect;
signaling pathway;
gonadotropin-releasing hormone receptor
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2017;33(6):554-559
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the effect and mechanism of Bushen Zhuyun formula(BSZYF)on gonadotropins and gonadotropin-releasing hormone receptor(GnRHR)signaling transduction in mifepristone-induced LPD rats,and to find the scientific evidence of"heart,kidney and uterus axis"theory of TCM.METHODS SD female rats were administered mifepris-tone suspension via oral gavage,followed by pharmaceutical intervention.Then,the serum follicle-stimulating hormone (FSH)and luteinizing hormone(LH)levels were detected by ELISA assay,the FSHβand LHβexpression levels in the pitui-tary were determined by qPCR,and the GnRHR expression levels were assessed by qPCR and Western blot analysis.Simulta-neously,transcription factors,such as c-Jun,Elk-1,Egr-1,Nur77 and CREB,and transduction molecules in PKC-MAPK, Ca2+-CAM,and cAMP-PKA signaling pathways,such as PKC,p38MAPK,ERK1/2,CAM,and PKA were assessed by qPCR and Western blot analysis.RESULTS After pharmaceutical intervention,BSZYF increased the serum FSH levels sup-pressed by mifepristone(P<0.05).Moreover,the expression of c-Jun,Elk-1,Egr-1,CREB,PKA,p38MAPK,JNK,ERK and PKA were was significantly upregulated in the BSZYF group compared with that in the mifepristone group(P<0.05). However,there was no statistical difference in Nur77 and CAM expression among the blank,mifepristone and BSZYF groups (P>0.05).CONCLUSION BSZYF can regulate abnormal pituitary gonadotropin secretion and GnRHR signaling transduc-tion systems induced by mifepristone,which confirms that the prescription can regulate the whole function of the reproductive axis,and explains the consistency of"Heart,kidney and uterus axis"theory in TCM and the concept of the hypothalamus-pitu-itary-ovary axis in modern medicine
