Effects of Classical Prescriptions of Blood-activating and Organ-purging Formula on Immunologic Barrier Function of Intestinal Mucosa in Rats with Postoperative Peritoneal Adhesion
10.14148/j.issn.1672-0482.2017.0519
- VernacularTitle:活血通腑方对术后腹腔粘连大鼠肠黏膜免疫屏障功能的影响
- Author:
Shuai YAN
1
;
Yin-Zi YUE
;
Li-Li YANG
;
Yao-Yao BIAN
;
Li ZENG
Author Information
1. 苏州市中医医院肛肠科
- Keywords:
peritoneal adhesion;
blood-activating and organ-purging formula;
mucosal immune barrier
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2017;33(5):519-523
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the effect of blood-activating and organ-purging formula in vivo of intestinal mucosal barrier after surgery in peritoneal adhesion.METHODS 60 male SD rats were randomly divided into control group,model group and Huoxue Tongfu Decoction group.Besides the control group,the rest of the rats were established with grater method,and the same dose of drugs were given at 3,6,12,24 h after operation.The blood and tissue samples were taken after 48 h.TNF-αlevels in plasma were determined by ELISA,sIgA in intestinal mucosa was detected by immunohistochemical method and the number of content of CD4 cells,lymphocytes apoptosis of intestinal mucosa were detected by TUNEL.RESULTS Compared with the control group,the plasma levels of TNF-αin the model group of 6,12,24 h showed significant difference(P <0.05～0.01),intestinal mucosa under lymphocyte were apoptosis significantly(P <0.01),12 h and 24 h secretion IgA significantly increased(P<0.05),and the model group 12 h,24 h and 3 h,6 h,submucous lymphocyte apoptosis decreased close to the level of control group,model group 3 h CD4 in intestinal mucosa cell number decreased significantly(P <0.01),there was no significant difference in the number of CD4 cells when three groups at 6 h,12 h and 24 h(P>0.05).Prescription in 3 h after operation can significantly reduce the levels of TNF-αin plasma of model rats increased number of intestinal mucosae,the content of IgA and CD4 cells decreased,submucous lymphocyte apoptosis.CONCLUSION Blood-activating and organ-purging formula group can reduce the blood level of TNF-alpha and submucous lymphocyte apoptosis,increase the number of intestinal mucosals content of IgA and CD4 cells,improving local immunity,inhibiting the inflammatory reaction.
