Characterization of Radix Isatidis Protein and its Ultrasound-assisted Extraction Process Optimization with Response Surface Meth-odology
10.14148/j.issn.1672-0482.2017.0504
- VernacularTitle:板蓝根蛋白的表征及响应曲面法优化其超声辅助提取工艺研究
- Author:
Ping XIAO
1
;
Hua-Lu LI
;
Wan-Ting CHEN
;
Xian-Wen ZHAO
;
Xian-Gui QIU
;
Xiang LI
;
Jian-Wei CHEN
;
Jin-Ao DUAN
Author Information
1. 南京中医药大学药学院
- Keywords:
Radix Isatidis protein;
Ultrasound-assisted extraction;
Response surface methodology;
Protein characterization
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2017;33(5):504-508
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To characterize the molecular weight of Radix Isatidis protein(RIP)and optimize the extraction process of protein from Radix Isatidis.METHODS Response surface methodology(RSM)was applied to optimize the extrac-tion of protein from Radix Isatidis.Extraction time,liquid-to-solid ratio and pH value were set as the investigated factors with respect to the protein yield.In addition,Design Expert software was used for data analysis.The RIP was characterized for composition using sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE).Scanning electron microscopy(SEM)analysis was performed to observe microstructure of the Radix Isatidis powder before and after ultrasound-assisted ex-traction(UAE).RESULTS Based on the RSM analysis,optimum conditions were determined as follows:twice ultrasonic ex-traction in 50 mmol/L Tris-HCl buffer solution,pH at 7.8,liquid-to-solid ratio at 80:1 and extraction for 65 min each time.Under the optimized conditions,the experimental values were 0.705％,which is in close agreement with values predicted by the model.The characterization of the RIP demonstrated that it contained five major groups of protein bands,namely bands of 19.2 kDa,21.5 kDa,24.8 kDa,34～43 kDa and >170 kDa respectively.CONCLUSION RSM can be applied for the optimi-zation of extraction process of RIP,which is effective,stable and feasible.
