Determination of the Related Components in Prepared Rhubarb Decoction and Its Pharmacodynamics Study
10.14148/j.issn.1672-0482.2017.0470
- VernacularTitle:熟大黄水煎液中化学成分含量测定及其药效学研究
- Author:
Hui ZHU
1
;
Xiao LIU
;
Ting-Ting ZHU
;
Xiao-Li WANG
;
Xiao-Chai ZHU
;
Bao-Chang CAI
Author Information
1. 南京中医药大学药学院
- Keywords:
Prepared rhubarb;
Assay;
HPLC;
Noxious heat blood stasis syndrome;
Hemorheology
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2017;33(5):470-475
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To develop an HPLC method for determination of main components in prepared rhubarb decoction and investigate its effects on rats with noxious heat blood stasis syndrome.METHODS The analysis was performed on a Hy-persil GOLD C18column with the mobile phase of 0.1％phosphoric acid solution(A)-acetonitrile(B).The detection wave-length was set at 254 nm and the column temperature was 30 ℃.The method was validated and the samples were determined under this condition.The abdominal aortic blood of rats with noxious heat blood stasis syndrome was collected and tested to obtain the hemorheological parameters.Also,the TXB2and 6-keto-PGF1αlevels in rats plasma were measured.RESULTS The developed HPLC method showed good linearity within certain concentration ranges.And the precision,repeatability,sta-bility and recovery all met the requirements.Compared with rats in model group,the whole blood viscosity at four shear rates(P<0.05),plasma viscosity(P<0.01)and the level of plasma fibrinogen content(P<0.01)in the high dose group were sig-nificantly decreased after doing,and the TT(P<0.01)and PT(P<0.01)values were significantly prolonged.Furthermore,the levels of TXB2(decreased,P<0.05)and 6-keto-PGF1α(increased,P<0.05)were significantly changed,and their ratio was corrected to be normal.CONCLUSION The method was simple,accurate and reliable,which could be used for quality control of prepared rhubarb decoction.Prepared rhubarb could improve vascular endothelial function by modifying blood rheol-ogy,promoting the secretion of PGI2,reducing the generation of TXA2and recovering the balance of TXB2/6-keto-PGF1α.
