Mechanism and Antibacterial Activity of Tibet Medicine Liuweidingxiang pills against Staphylococcus aureus
10.14148/j.issn.1672-0482.2017.0408
- VernacularTitle:藏药六味丁香丸对金黄色葡萄球菌的抑菌作用及机制探讨
- Author:
Ping HE
1
;
Xiao-Li ZHU
;
Tsering DAWA
;
Shi-Hua WANG
;
Shu-Mei HE
;
Dong-Ya YUAN
;
Jin-Bo WANG
Author Information
1. 西藏民族大学医学院
- Keywords:
Tibetan medicine;
Liuweidingxiang Pills;
Staphylococcus aureus;
antibacterial activity;
the minimal inhibitory concentration
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2017;33(4):408-411
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate antibacterial activity and mechanism of Tibetan medicine Liuweidingxiang pills against Staphylococcus aureus,and provide theoretical basis for clinical application.METHODS M-H agar-punching method was adopted to research antibacterial activity of Liuweidingxiang pills against Staphylococcus aureus,and the minimum inhibitory concentration (MIC) was measured by broth dilution method.The absorbance OD600nm of culture medium mixed with medicine was continuously recorded for 36h and growth curve of S.aureus was drawn.Furthermore,the antibacterial mechanism was explored using scanning electron microscopy and cell wall permeability test.RESULTS The diameter of inhibition zone was (21.8±2.36) mm at 800 mg/mL,and the MIC was between 12.5 mg/mL and 25 mg/mL.This medicine had significant inhibition to bacterial proliferation.The structure of bacterial cell was changed and the permeability of cell wall was increased.CONCLUSION Liuweidingxiang pills had significant antimicrobial effect on multidrug-resistant S.aureus,which implied potential value in clinical application.
