Study on Antiblastic Effect Mechanism of ER(+) Human Breast Cancer MCF-7 Cells by Triptolide Based on CYP19

VernacularTitle:基于CYP19探讨雷公藤甲素抗ER(+)人乳腺癌MCF-7细胞作用机理
Author: Min LIU ¹ ; Lu-Rong ZHANG ; Guo-Qiang LIANG ; Dun-Yu JIAN ; Xiao-Di ZHANG
Author Information

1. 南京中医药大学附属苏州市中医医院
Keywords: CYP19; triptolide; human breast cancer MCF-7 Cells; transfaction
From: Journal of Nanjing University of Traditional Chinese Medicine 2017;33(4):391-394
CountryChina
Language:Chinese
Abstract: OBJECTIVE To investigate the mechanisms of Triptolide in ER(+) human breast cancer MCF-7 cells from CYP19.METHODS The inhibition of the MCF-7 cells influenced by Triptolide and Letrozole was analyzed respectively by MTT assays.The aromatase levels were measured by Western blot.The CYP19 gene was observed by RT-PCR.The MCF-7 cells of high and low expression CYP19 gene were transfacted.It's CYP-19 and downstream channel gene JNK,p-38 and ERK were observed by Western blot.RESULTS Triptolide displayed a dose-and time-dependent inhibition of the MCF-7 cells.It inhibited the aromatase and CYP19.The expression of CYP19 in MCF-7 cells of low expression CYP19 was significantly decreased.Phosphorylation of JNK,p38 and ERK was inhibited too.CONCLUSION Triptolide inhibit MCF-7 cells not only via inhibition aromatase,but also blocking Ras-Raf-MAPK-ERK enzyme.