Preparationof Doxorubicin-Copper-Curcumin Nano-micelles and its In-vitro Activities Evaluaion
10.14148/j.issn.1672-0482.2017.0308
- VernacularTitle:阿霉素-铜-姜黄素纳米胶束的制备及体外性质研究
- Author:
Jing CHEN
1
;
Hong-Zhi QIAO
;
Ya-Ping CHAI
;
Xin-Yi DAI
;
Juan SUN
;
Qing-Long DING
;
Liu-Qing DI
Author Information
1. 南京中医药大学药学院
- Keywords:
curcumin;
doxorubicin;
pH-sensitive;
coordination;
micelles
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2017;33(3):308-312
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE With Poloxamer 407(P407) as the carrier, doxorubicin-copper-curcumin ternary complex was encapsulated in the micelles to achieve quantitative and intelligent drug release behavior and synergistic effect.METHODS With P407 as the carrier,the preparing technology was optimized by Box-Behnken design and the in vitro pH-sensitive drug release behavior of DOX-Cu-Cur/P407 was examined;the MTT analysis was carried out to evaluate the cytotoxicity to tumor cells.RESULTS Under the optimum condition, the encapsulation efficiency of doxorubicin and curcumin was (93.17 ± 1.05)% and (100.03 ± 1.10)%, size distribution was (31.06 ± 4.20) nm,PDI is 0.174 ± 0.028.Meantime, in vitro drug release test showed the DOX-Cu-Cur/P407 micelles was pH-sensitive.The MTT analysis showed DOX-Cu-Cur/P407 micelles could significantly inhibit the tumor cells with a better synergistic effect compared with free drugs.CONCLUSION The optimal DOX-Cu-Cur/P407 micelle is uniform in size and has satisfactory encapsulation efficiency.Meanwhile it is sensitive to acidic microenvironment of tumor and can achieve the purpose of quantitative and intelligent drug release.
