Protective Effects and Mechanism of Polysaccharide from Platycodon Grandiflorum on Damage of PC12 Cells Induced by H2O2

VernacularTitle:桔梗多糖对H2O2诱导PC12细胞氧化损伤的保护作用及机制研究
Author: Cheng-Yuan GU ¹ ; Qiu-Li CHEN ; Hai-Tao LI
Author Information

1. 南京中医药大学药学院
Keywords: Platycodon grandiflorum polysaccharide; antioxidation; PC12 cells; hydrogen peroxide; NOX2
From: Journal of Nanjing University of Traditional Chinese Medicine 2017;33(3):268-272
CountryChina
Language:Chinese
Abstract: OBJECTIVE To determine the protective effects of Platycodon grandiflorum polysaccharide (PGP) on PC12 cells injured by H2O2 and the underlying mechanisms.METHODS The cell viability was analyzed by MTT assay, the model was established by treating PC12 cells with H2O2.The levels of LDH, MDA, SOD and GSH-Px were determined by Kits.ROS production was determined by DCFH-DA fluorescence.mRNA and proteins of NOX2, p22phox and Rac were analyzed by qPCR and Western Blot methods.RESULTS Compared with the model, treatment with PGP significantly decrease of LDH, MDA and intracellular ROS production, as well as increase activity of SOD and GSH-Px.Furthermore, PGP could down-regulate mRNA and proteins of NOX2, p22phox and Rac.CONCLUSION PGP exhibited protective effect on PC12 cells injury induced by H2O2, it may via the suppression of NOX2 activation.