Determination of 22 elements in whole blood by inductively coupled plasma mass spectrometry
10.3760/cma.j.cn121094-20240208-00055
- VernacularTitle:全血中22种元素测定的电感耦合等离子体质谱法
- Author:
Yan LI
1
;
Panliang NING
;
Yueming JIANG
Author Information
1. 广西壮族自治区职业病防治研究院化学中毒与重金属检测中心，南宁 530021
- Keywords:
Elements;
Whole blood;
Mass spectrometry;
Inductively coupled plasma mass spectrometry, ICP-MS
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2024;42(9):695-699
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the simultaneous determination of 22 elements, including beryllium, vanadium, chromium, manganese, iron, calcium, magnesium, barium, cobalt, cadmium, copper, zinc, arsenic, selenium, titanium, strontium, nickel, molybdenum, tin, antimony, thallium and lead, in whole blood by inductively coupled plasma mass spectrometry (ICP-MS) .Methods:In September 2023, the analysis conditions were determined by optimizing the detection mode of the instrument, the pretreatment mode and the dilution factor of the samples, etc. Whole blood samples were diluted with a mixture of 0.1% nitric acid and 0.05% triton X-100, and centrifuged at 2000 r/min by high-speed centrifuge for 2 min. The supernatant was taken into inductively coupled plasma mass spectrometer to determine the content of 22 elements, and the detection limit and precision of the method were analyzed.Results:The 22 elements had a good linear relationship in their respective measurement ranges ( r=0.9991-0.9999), the detection limit ranged from 0.003 μg/L to 0.012 mg/L. The intra-batch precision ranged from 0.5% to 7.2%, the inter-batch precision ranged from 0.4% to 9.4%, and the average recoveries ranged from 80.6% to 114.9%. Conclusion:ICP-MS method has a good effect on the determination of 22 elements in whole blood. The method is fast and simple, and can be used for clinical detection of multiple elements in whole blood.
