Biomarkers in Urine and Feces of BALB/c Mice Infected with RSV Pneumonia Using GC-MS Technology

VernacularTitle:基于GC-MS代谢组学的RSV肺炎小鼠尿液和粪便生物标记物研究
Author: Xin MENG ¹ ; Shou-Chuan WANG ; Tong XIE ; Jian-Ya XU ; Cun-Si SHEN ; Jin-Jun SHAN
Author Information

1. 江苏省儿童呼吸疾病(中医药)重点实验室
Keywords: GC-MS; urine; feces; RSV pneumonia; biomarkers
From: Journal of Nanjing University of Traditional Chinese Medicine 2017;33(2):198-206
CountryChina
Language:Chinese
Abstract: OBJECTIVE To detect the relative abundance changes of metabolites in urine and feces of BALB/c mice to seek the biomarkers that contribute to the diagnosis by GC-MS technology.METHODS BALB/c mice were challenged intranasally to establish the RSV pneumonia models.Urine and feces were collected respectively,oximated,derivatized and detected by GC-MS.RESULTS 38 metabolites in urine were detected,among which lactic acid,propanoic acid and L-proline were statistical-ly significant(P<0.05).Moreover,43 metabolites in feces were detected,and leucine,aspartic acid,cystine,tyrosine and pyrimidine had statistical significance(P<0.05).CONCLUSION GC-MS technology is feasible for seeking biomarkers in u-rine and feces of mice infected with RSV pneumonia.