A Metabolomics Study in Pediatric Viral Pneumonia with Syndrome of Wind-Cold
10.14148/j.issn.1672-0482.2017.0193
- VernacularTitle:儿童病毒性肺炎风寒郁肺证代谢组学研究
- Author:
Jing REN
1
;
Shou-Chuan WANG
;
Qiu-Yue ZHANG
;
Wen-Sheng ZHAI
;
Zi-Juan LAI
;
Jin-Jun SHAN
Author Information
1. 江苏省儿童呼吸疾病(中医药)重点实验室
- Keywords:
Metabolomics;
viral pneumonia in children;
syndrome of wind-cold;
GC-MS
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2017;33(2):193-197
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE Discussion on the application of GC-MS to study the metabolic fingerprinting of serum in pneumo-nia group with syndrome of wind-cold in order to provide data support for the quantitative study of syndromes in TCM.METH-ODS In the study of patients with wind-cold(WC)in 30 cases,the normal healthy(NH)in 30 cases,Serum samples were col-lected and serum metabolites profiles were analyzed by GC-MS coupled with a couple of statistical analyses.RESULTS Clear separations between WC and NH existed.Differential metabolites and several metabolic pathways related organic acids,amino acids,fat were identified.Compared with NH group,the levels of organic acids,amino acids,fat were upregulated,such as L-Alanine,L-Aspartic acid,L-Glutamic acid,Glycine,Serine,L-Threonine,Taurine,L-Lysine,and D-2-Aminobutyric acid, Glycolic acid,Glycerol,1-Monopalmitin,cholesterol,d-Glucose were down in serum with WC group.And,five abnormal met-abolic pathways,such as Alanine metabolism,Glycine metabolism,Taurine metabolism,aa-tRNA biosynthesis and Lysine deg-radation were identified.CONCLUSION This study detected abnormal metabolites and pathways in WC,which may be due to multiple energy metabolism pathways-virus infected;taking advantage of metablomic techniques,we may establish Character-ized Spectrum Database in pediatric viral pneumonia and syndrome differentiation pattern standardization of TCM so as to pro-vide the useful methods in study of syndrome standardization.
