Acetylated Modification of Polysaccharides from Stems and Leaves of Abelmoschus Manihot and Its Immunoregulatory Activity
10.14148/j.issn.1672-0482.2017.0167
- VernacularTitle:黄蜀葵茎叶多糖的乙酰化修饰及其免疫调节活性研究
- Author:
Xin-Xin PAN
1
;
Shu JIANG
;
Yue ZHU
;
Da-Wei QIAN
;
Hui YAN
;
Jin-Ao DUAN
Author Information
1. 南京中医药大学
- Keywords:
Abelmoschus manihot;
polysaccharide;
acetylation;
immunomodulatory activity;
resource utilization
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2017;33(2):167-172
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE Acetylated modification was applied to modify the structure of polysaccharide from stems and leav-es of Abelmoschus manihot,and the in vitro immunoregulatory activity of polysaccharides were evaluated,which was expected to provide the scientific evidence for the resource utilization of A.manihot disused parts.METHODS The crude polysaccha-ride(SLAMP)was obtained by water extraction and alcohol precipitation,and neutral polysaccharide(SLAMP-a)was further prepared by DEAE-52 ion exchange column.Acetic anhydride was used as acetylating agent to synthesize three acetylated de-rivatives (SLAMP-a1 ,SLAMP-a2 and SLAMP-a3).Their structures were preliminarily identified by chemical composition a-nalysis,pre-column derivatization HPLC method and IR spectrum.In addition,in vitro immunoregulatory activity of SLAMP-a and three acetylated derivatives was evaluated by splenocyte proliferation together with its effects on NO production of RAW264.7.RESULTS SLAMP-a showed little immunomodulatory activity and the total sugar content was 99.76%;Among the three derivatives,only SLAMP-a1 could significantly stimulate the proliferation of spleen cells and activate RAW264.7 to product NO.SLAMP-a1 mainly contained glucose with a few of mannose,galactose and arabinose,and the total sugar and DS was 82.50% and 0.62 respectively.CONCLUSION In vitro immunomodulatory activity of polysaccharide SLAMP-a from stems and leaves of A.manihot is significantly enhanced by acetylation and SLAMP-a1 possesses the potential to develop as immunoregulatory agents.The acetylation modification is an effective way for resource utilization of polysaccharide from the disused stems and leaves of A.manihot.
