Effect of Huangqi Baoxin Decoction on Ventricular Remodeling in Rats with Dilated Cardiomyopathy Induced by Adriamycin
10.14148/j.issn.1672-0482.2017.0160
- VernacularTitle:黄芪保心汤对阿霉素诱导扩张型心肌病大鼠心室重构的影响
- Author:
Lei LU
1
;
Pei-Ying ZHANG
;
Yong-Gang CHEN
;
Xiao-Dan LIU
Author Information
1. 南京中医药大学第一临床医学院
- Keywords:
Huangqi Baoxin Decoction;
dilated cardiomyopathy;
adriamycin;
heart function
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2017;33(2):160-166
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To preliminary discuss the influence and mechanism of Huangqi Baoxin Decoction on ventricular remodeling in rats with dilated cardiomyopathy(DCM)induced by adriamycin.METHODS 90 SD rats were given intraperito-neal inj ection of adriamycin to establish the DCM model,with 55 rats model being succeed.Then they were randomly divided into model group(the same volume of physiological saline solution),low-dose group with Huangqibaoxin Decoction(5.67 g/kg),middle-dose group with Huangqibaoxin Decoction(11.34 g/kg),high-dose group with Huangqibaoxin Decoction(22.68 g/kg),and captopril group(6.75 mg/kg of captopril),with 11 rats in each group.The rats were given orally administrated drugs once a day,with 10 rats in the normal control group being treated with the same volume of physiological saline solution. 4 weeks later,rats in each group went through the echocardiography to access the construction and function of their left ven-tricular.Crotid artery intubation was applied to monitor their hemodynamic indxes,with changes of myocardial tissue mor-phology being observed.Besides,NT-proBNP,ST2,AngⅡ,ALD and Renin were tested through ELISA,together with the activity of SOD,MDA,and LDH being tested via colorimetric method.RESULTS Compared with the normal group,the LVEDD and LVESD in rats of the model group were increased,with EF and FS being decreased(P<0.01).The LVDP,±dp/dtmax went down,while the LVEDP went up(P<0.01).The heart weight index increased(P<0.01),with the myocardialcells being arranged in disorder and massive hyperplasia of fibrous tissue.The NT-proBNP,ST2,Renin,AngⅡ and ALDlevel increased(P<0.01).The level of SOD decreased,while the content of LDH and MDA increased(P<0.01).Comparedto the model group,LVEDD and LVESD levels in the low,middle and high dose group of Huangqi Baoxin Decoction were reducedto varying degrees,with increased EF and FS(P<0.05~0.01).They experienced an increased LVDP,±dp/dtmax anddecreased LVEDP(P<0.05~0.01),decreased HWI and LVMI(P <0.05~0.01).And the myocardial cells were neatly arranged,the hyperplasia of fibrous tissue were relived.Besides,the levels of NT-proBNP,ST2,Renin,AngⅡ and ALD wentdown on diverse degree(P<0.05~0.01),with decreased LDH and MDA and increased SOD(P<0.05~0.01).There was adose-effect relationship among the groups of different doses towards the improvement on the above indexes.CONCLUSIONHuangqi Baoxin Decoction can improve the cardiac hypertrophy and fibrosis in rats with dilated cardiomyopathy.It also improvesthe cardiac function and inhibits ventricular remodeling by regulating the oxidation factors and inhibiting the activation ofRAAS neuroendocrine factors.
