The Effects of Raddeanin A on Human Gastric Cancer BGC-823 Cell Cycle and STAT3 Signaling Pathway
10.14148/j.issn.1672-0482.2016.0078
- VernacularTitle:竹节香附素A对人胃癌细胞株BGC-823细胞周期及STAT3信号通路的影响
- Author:
Gang XUE
1
;
Xi ZOU
;
Jin-Yong ZHOU
;
Rui-Ping WANG
Author Information
1. 如皋市中医院肿瘤科
- Keywords:
Raddeanin A;
human gastric cancer cell BGC-823;
cell cycle;
STAT3 signaling pathway
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2017;33(1):78-81
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of Raddeanin A(RA) on cell cycle and STAT3 signaling pathway in hu-man gastric cancer cell line BGC-823.METHODS Effects of different concentrations of RA on proliferation of BGC-823 cell line were investigated with MTT assay;flow cytometry was used to detect the cell cycle inhibition;wounding heal assay was used to measure the influence of migration ability of RA on BGC-823 cell;the effect of STAT3 signaling pathway of RA on BGC-823 cell was measured by Western blot.RESULTS After the BGC-823 cells were treated by RA for 12,24,48 h,com-pared with control group,RA significantly inhibited the proliferation of BGC-823 cell (P <0.01).The inhibitive effects pres-ented a dose and time-dependent manner.Flow cytometry demonstrated that RA blocked the cell cycle in S phase;wounding heal assay revealed that RA inhibited the migration of BGC-823 cell.Western blot results showed that RA down-regulated the expression of phosphorylated STAT3 (p-STAT3).CONCLUSION RA can effectively inhibit the proliferation of BGC-823 cell,block its cell cycle in S phase and inhibit the expression of p-STAT3.
