Liuwei Dihuang Pills Attenuates Liver Fibrosis by Inhibiting Macrophage Activation in CCl4 Induced Liver Injury
10.14148/j.issn.1672-0482.2016.0065
- VernacularTitle:六味地黄丸抑制巨噬细胞激活抗肝纤维化作用机制研究
- Author:
Xue-Min WANG
1
;
Ya-Qin CUI
;
Jing WANG
;
Bing-Ye SHI
;
U Xin-j LI
Author Information
1. 河北大学附属医院
- Keywords:
liver fibrosis;
Liuwei Dihuang Pills;
macrophage
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2017;33(1):65-68
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To observe the inhibitory effects of Liuwei Dihuang Pills(LWDHP) on macrophages activation in CCl4-induced liver fibrosis in mice.METHODS C57BL/6 mice were induced liver fibrosis by CCl4 exposure and administered with LWDHP for 6 weeks simultaneously.Liver tissue was investigated by HE and Sirius red staining.α-SMA was analyzed by immunofluorescence,qPCR and Western blot.Liver macrophages were observed by immunohistochemistry of CD68.The pro-inflammatory cytokines and chemokine such as TNF-α,IL-1β,CXCR3 and MCP1 were detected by qPCR or Western blot analysis.RESULTS After 6 weeks of CCl4 administration,the expression ofα-SMA significantly increased,and LWDHP po-tently inhibited the α-SMA expression.Immunohistochemistry showed that the expression of CD68 was very weak in normal group,CD68 was distributed mainly between fibrotic septa with strong positive expression in CCl4 model group;Real-time quantitative PCR showed that TNF-α,IL-1β,MCP1 and CXCR3 expression significantly increased in model group compared with normal group.Compared with the model group,LWDHP significantly reduced the expression of CD68,inflammatory fac-tors and chemotactic factors.CONCLUSION LWDHP shows a potent inhibition of macrophage activation in CCl4-induced liv-er fibrosis.
