The Effect and Mechanism of Renshen Pingfei Prescription in the Pulmonary Fibrosis Model Induced by Silica in Rats
10.14148/j.issn.1672-0482.2017.0049
- VernacularTitle:人参平肺方对二氧化硅所致大鼠肺纤维化的作用及机制探讨
- Author:
Kang XU
1
;
Hui-Qin XU
;
Xin-Sheng FAN
;
Yu-Hao FAN
;
Hong-Sheng SHENG
;
Guo-Ying DAI
;
Peng-Li WANG
Author Information
1. 南京中医药大学药学院
- Keywords:
pulmonary fibrosis;
Renshen Pingfei prescription;
Silica;
TGF-β1
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2017;33(1):49-53
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To research the effect of Renshen Pingfei prescription in the pulmonary fibrosis model induced by Silica in rat and discuss the possible mechanism.METHODS SD rats were anesthetized and injected with silica dust suspension into trachea to establish pulmonary fibrosis model,then randomly divided into model group,prednisone group,Renshen Pingfei prescription 3.0,6.0,12.0 g/kg dose group,and establish normal group.Every day on time,the intragastric administration were proceeded for 48 days and lung tissue of rats were weighed to calculate pulmonary coefficient.Evaluating the inflammation and fibrosis of pulmonary alveoli through HE and Masson HE staining.The content of hydroxyproline(HYP) in lung tissue was detected by assay kit.The level of TGF-β1,NF-κB,PDGF,TNF-αand IFN-γin alveolar lavage fluid were detected using ELISA-kit and the protein expression level of TGF-β1,Samd2/3 and ERK1/2 in lung were detected through immunohisto-chemical staining.RESULTS The kidney coefficient,the inflammation and fibrosis of pulmonary alveoli,the level of TGF-β1, NF-κB,PDGF,and TNF-αin alveolar lavage fluid the protein expression level of TGF-β1,Samd2/3 and ERK1/2 in lung were significantly decreased(P <0.05)in the pulmonary fibrosis model of rat after administration with Renshen Pingfei prescription, otherwise the level of IFN-γin alveolar lavage fluid were significantly increased(P <0.05).CONCLUSION Renshen Pingfei prescription has protective effect in pulmonary fibrosis and its mechanism may relate to TGF-β/Smad/ERK signal.
