Purification,Structural Characterization and Immunomodulatory and Antitumor Activities of a Polysaccharide Isolated from Mo-mordica Charantia L.
10.14148/j.issn.1672-0482.2017.0033
- VernacularTitle:苦瓜多糖的纯化、结构解析及其免疫调节和抗肿瘤活性研究
- Author:
Yi ZHANG
1
;
Wang WANG
;
Yin CAI
;
Min WANG
;
Chun-Yan GU
;
Ye YANG
Author Information
1. 南通大学附属医院烧伤整形外科
- Keywords:
polysaccharide from Momordica charantia L.;
purification;
structural characterization;
immunomodulatory ac-tivity;
antitumor
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2017;33(1):33-39
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To isolate and purify an active polysaccharide of Momordica charantia L.and investigate its struc-tural characterization and immunomodulatory and antitumor activities.METHODS A polysaccharides was isolated from Mo-mordica charantia L.in hot water extraction followed by dialysis and purification through anion exchange cellulose chromatog-raphy and gel filtration chromatography,and its molecular weight was measured by high performance gel permeation chroma-tography.Additionally,Fourier transform infrared spectroscopy(FT-IR)and nuclear magnetic resonance(NMR)were used to determine its partial structural characterization,and its immunomodulatory and antitumor activities were measured by cell and molecular biological techniques and pharmacological methods.RESULTS A heteropolysaccharide named MCP-2 with molecu-lar weight of 745 kDa was isolated and purified,which was shown to be consisted of rhamnose,galacturonic acid,galactose, xylose,and arabinose with corresponding molar ratio being 1.1 9 ∶ 1 5.97 ∶ 3.40 ∶ 0.73 ∶ 0.94.Furthermore,MCP-2 was found to promote the lymphocyte proliferation of normal mice and to raise the levels of NO,IL-1β and TNF-α produced by macrophage RAW264.7.Intriguingly,MCP-2 presented the antitumor activity against S180 cells in vivo as well.CONCLU-SION All experimental data showed that MCP-2 had apparent immunologic enhancement and antitumor activities.
