Simultaneously Determination of Eight Components in Schisandra chinensis Georgi by UPLC-MS/MS
10.14148/j.issn.1672-0482.2016.0585
- VernacularTitle:UPLC-MS/MS 法同时测定黄芩中8种主要指标性成分的含量
- Author:
Tong XIE
1
;
Jian-Ya XU
;
Cun-Si SHEN
;
Liu-Qing DI
;
Shou-Chuan WANG
;
Un Jin-j SHAN
Author Information
1. 江苏省儿童呼吸疾病 中医药 重点实验室
- Keywords:
UPLC-MS/MS;
MRM;
Schisandra chinensis Georgi
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(6):585-588
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To develop a sensitive and selective ultra performance liquid chromatography-triple quadrupole mass spectrometer(UPLC-MS/MS) method for quantification of eight components(chrysin,baicalein,apigenin,orohylin A, wogonin,baicalin,wogonoside,oroxin A) in Schisandra chinensis Georgi.METHODS The separation was performed on a Thermo BDS Hypersil C1 8(2.1 mm×100 mm,2.3 μm) column.The mobile consisted of acetonitrile (A) and 0.1% formic acid aqueous with a gradient elution at a flow-rate of 0.25 mL/min.The detection was acquired by MRM.The column temper-ature was 40 ℃.RESULTS The linear ranges of chrysin,baicalein,apigenin,orohylin A,wogonin,baicalin,wogonoside, oroxin A were 0.975~62.5 ng/mL(r 2 =0.999 8),4.875~2 500 ng/mL(r 2 =0.999 7),0.975~62.5 ng/mL(r 2 =0.999 4), 0.097 5~12.5 ng/mL(r 2 =0.999 6),0.048 83~6.25 ng/mL(r 2 =0.999 9),4.875~2 500 ng/mL(r 2 =0.999 8),0.488 3~250 ng/mL(r 2 =0.999 9),0.488 3 ~ 250 ng/mL(r 2 = 0.999 8),respectively.The recoveries were 98.75%,96.44%, 101.13%,99.41%,102.26%,98.45%,96.75%,99.42%.CONCLUSION The proposed method enables quantification for the study of the compounds of chrysin,baicalein,apigenin,orohylin A,wogonin,baicalin,wogonoside,oroxin A in Schisan-dra chinensis Georgi.
