The Hypoglycemic Activity of Homogeneous Polysaccharides from Dendrobium Officinale
10.14148/j.issn.1672-0482.2016.0566
- VernacularTitle:铁皮石斛多糖降血糖作用研究
- Author:
Zhi-Yuan TANG
1
;
Xiao-Yu ZHOU
;
Jian FENG
;
Hua LIU
;
Jun LI
;
Yi WU
;
Xu-Mei YE
;
Song-Shi NI
Author Information
1. 南通大学附属医院
- Keywords:
Dendrobimn officinale;
isolation and purification;
monosaccharide composition;
hypoglycemic activity
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(6):566-570
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To characterize the homogeneous polysaccharides from Dendrobium officinale Kimura et Migo and study the hypoglycemic activity of the isolated polysaccharides.METHODS DEAE-cellulose anion-exchange chromatography was used to isolate homogeneous polysaccharides from crude polysaccharides (DOP);Monosaccharide composition was ana-lysed after trimethylsilation;and the chemical structure of isolated polysaccharide was characterized with infrared spectroscopy;The effects of isolated polysaccharides on the levels of fasting blood glucose,plasma insulin,and glycosylated serum protein were estimated using diabetic mice models induced by alloxan.RESULTS Four isolated polysaccharides were isolated from DOP,in which DOP1 had the highest content.DOP1 was composed of arabinose,galactose,glucose and mannose in a molar ratio of 3.5∶1.2∶1.5∶1.0.The infrared spectroscopy of various isolated polysaccharides showed that they all had the obvi-ous characteristic absorption peaks of corresponding monosaccharides.The levels of fasting blood glucose and glycosylated ser-um protein decreased obviously after a 21 day ig administration in diabetic mice models(P <0.01),in addition,all the four iso-lated polysaccharides increased glycosylated serum protein levels in varying degrees after a 21 day dosage (P <0.05 ~0.01). DOP1 displayed the optimal effects on the improvements of above three biochemistry indexes compared with the other three i-solated polysaccharides.CONCLUSION Four isolated polysaccharides from DOP displayed hypoglycemic activity in diabetic mice models induced by alloxan.
