Effect of Bushen Huoxue Jiangu Decoction Drug Serum on Osteoblast Proliferation,Differentiation,Maturation and Mineralization in Vitro
10.14148/j.issn.1672-0482.2016.0561
- VernacularTitle:补肾活血健骨方含药血清对人成骨细胞增殖、分化及矿化功能的影响
- Author:
Ju-Peng CHEN
1
;
Gui-Cheng HUANG
;
Wei-Dong LI
;
Hua-Dong LIU
;
Run-Lin XING
Author Information
1. 南京中医药大学附属医院
- Keywords:
Bushen Huoxue Jiangu Decoction;
posemenopausal osteoporosis;
drug serum;
osteoblast;
proliferation and dif-ferentiation
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(6):561-565
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To observe the effects of Bushen Huoxue Jiangu Decoction drug serum on osteoblast proliferation, differentiation,maturation and mineralization in vitro ,which will provide the theoretical basis for osteoporosis treatment. METHODS Cancellous bone specimens from the femoral head were obtained from patients with femoral neck fracture merger osteoporosis,and the osteoblast cells were seeded and cultured in vitro to establish the osteoblast differentiation model.High, middle and low doses of Bushen Huoxue Jiangu Decoction drug serum were prepared.MTT method,ALP activity assay and a-lizarin red staining were used to observe the proliferation,activity of ALP and the number of mineral node of cultured osteo-blasts in vitro .RESULTS MTT assay displayed different degrees of osteoblast proliferation in high and middle dose groups and showed some dose dependence after 48 h of drug intervention.The activities of ALP in high,middle and low dose groups obviously increased after 72 h of drug intervention.The deposition of calcium salt crystal in alizarin red staining obviously in-creased in high dose group after 72 h of drug intervention.CONCLUSION Bushen Huoxue Jiangu Decoction drug serum can promote osteoblast proliferation,differentiation,maturation and mineralization in vitro .
