6'-O-Galloylpaeoniflorin Protects Human Keratinocytes Against Oxidative Stress-Induced Cell Damage.
- Author:
Cheng Wen YAO
1
;
Mei Jing PIAO
;
Ki Cheon KIM
;
Jian ZHENG
;
Ji Won CHA
;
Jin Won HYUN
Author Information
1. School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 690-756, Republic of Korea. jinwonh@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
6'-O-Galloylpaeoniflorin;
HaCaT keratinocyte;
Hydrogen peroxide;
Oxidative stress;
Reactive oxygen species
- MeSH:
Cell Death;
DNA;
Far East;
Flowers;
Herbal Medicine;
Humans*;
Hydrogen Peroxide;
Hydroxyl Radical;
Keratinocytes*;
Oxidative Stress;
Paeonia;
Plants;
Reactive Oxygen Species;
Superoxides
- From:Biomolecules & Therapeutics
2013;21(5):349-357
- CountryRepublic of Korea
- Language:English
-
Abstract:
6'-O-galloylpaeoniflorin (GPF) is a galloylated derivate of paeoniflorin and a key chemical constituent of the peony root, a perennial flowering plant that is widely used as an herbal medicine in East Asia. This study is the first investigation of the cytoprotective effects of GPF against hydrogen peroxide (H2O2)-induced cell injury and death in human HaCaT keratinocytes. GPF demonstrated a significant scavenging capacity against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, H2O2-generated intracellular reactive oxygen species (ROS), the superoxide anion radical (O2-), and the hydroxyl radical (*OH). GPF also safeguarded HaCaT keratinocytes against H2O2-provoked apoptotic cell death and attenuated oxidative macromolecular damage to DNA, lipids, and proteins. The compound exerted its cytoprotective actions in keratinocytes at least in part by decreasing the number of DNA strand breaks, the levels of 8-isoprostane (a stable end-product of lipid peroxidation), and the formation of carbonylated protein species. Taken together, these results indicate that GPF may be developed as a cytoprotector against ROS-mediated oxidative stress.
