Adjustment of Moxibustion on Serum Level of IL-1 7,RANKL and TGF-βin RA Rats
10.14148/j.issn.1672-0482.2016.0543
- VernacularTitle:艾灸调控 IL-17对实验性 RA 大鼠血清 RANKL 和TGF-β含量的影响
- Author:
Zi-Shan GAO
1
;
Jun-Xue GAN
;
Jiang-Yan GUO
;
Shu-Ping FU
;
Ke GAN
Author Information
1. 南京中医药大学第二临床医学院
- Keywords:
moxibustion;
RA;
IL-1 7;
TGF-β;
RANKL;
bone destruction
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2016;32(6):543-547
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the IL-1 7 signal transduction mechanism of anti-inflammatory effect of moxibustion on RA rats.METHODS 66 SD rats were randomly assigned to control,model,control+moxa,moxa,IL-1 7 overexpression+moxa,IL-1 7 knockdown+moxa,IL-1 7 overexpression and IL-1 7 knockdown groups.Complete freund's adjuvant were injected into the right paw of SD rats to establish RA model,and siRNAs were injected for IL-1 7 overexpression/knockdown models. After 7 d,moxibustion treatments were performed for 18 d.RESULTS The swelling degree of RA rats and the serum level of IL-1 7 and RANKL were significantly higher than control group(P <0.01),and the PWTL of RA rats,serum level of TGF-βwere significantly lower than control group.After 18 d of moxibustion treatment,the swelling degree of RA rats and the serum level of IL-1 7,RANKL were significantly lower than control group(P <0.01),and the PWTL of RA rats and serum level of TGF-βwere significantly higher than control group.In addition,the serum level of RANKL in IL-1 7 knockdown+moxa rats were significantly higher than moxa group(P <0.01);the serum level of RANKL in IL-1 7 knockdown+moxa rats were signif-icantly lower than moxa group and control group(P < 0.01).CONCLUSION Moxibustion may adjust the serum level of RANKL and TGF-βby supressed the serum level of IL-1 7 therefore relieving RA in RA rats.
